Rapid glucose concentration detection utilizing disposable integrated microfluidic chip |
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Authors: | Hui-Hsiung Hou Yao-Nan Wang Chin-Lung Chang Ruey-Jen Yang Lung-Ming Fu |
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Affiliation: | (1) Department of Engineering Science, National Cheng Kung University, Tainan, 70101, Taiwan;(2) Department of Vehicle Engineering, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan;(3) Department of Materials Engineering, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan; |
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Abstract: | A novel three-dimensional (3D) disposable glucose concentration detection chip is presented. The chip comprises a four-layer
polymethyl methacrylate (PMMA) structure and is fabricated using a commercial CO2 laser and a hot-press bonding technique. In the proposed device, the glucose solution is injected into a double parallel
connection micromixer (DPCM) and is mixed with DNS reagent by means of a self-rotation effect. An experimental platform has
been created for multiple reaction process by integrating chip and micro-heater. The fluid streams exiting the two circular
mixing chambers of the DPCM are then combined and mixed further at a T-type microchannel outlet before passing to a collection
chamber. Numerical simulations are performed to analyze the vortex streamline distribution within the DPCM and to estimate
the mixing performance. The numerical results show that a mixing efficiency as high as 92.5% can be obtained at low Reynolds
numbers (Re = 12). It is found a good linear relation of R
2 = 0.9953 from the chip detection method comparing to the traditional method of R
2 = 0.9976 at DNS reagent and glucose solution volume ratio of 1:1. In addition, the experimental results show that the accuracy
of the glucose concentration measurements obtained using the proposed microfluidic chip is comparable with that of the measurements
obtained using a conventional large-scale detection method. Overall, the results presented in this study indicate that the
DPCM chip provides a rapid and low-cost means of detecting the concentration of glucose solutions. |
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