| 海藻酸钠-壳聚糖共固定化菠萝茎蛋白酶的研究 |
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| 引用本文： | 高尚欣,周冰如,周锦丝,李燕菲,赵力超.海藻酸钠-壳聚糖共固定化菠萝茎蛋白酶的研究[J].食品工业科技,2011(5):209-211. |
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| 作者姓名： | 高尚欣 周冰如 周锦丝 李燕菲 赵力超 |
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| 作者单位： | 华南农业大学食品学院,广东广州,510000 |
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| 摘 要： | 以海藻酸钠、壳聚糖为材料共固定化菠萝茎蛋白酶,并对固定化条件以及固定化酶的酶学特性进行了研究。通过单因素及正交实验确定固定化酶的最佳条件为:酶用量0.4mg/g、海藻酸钠浓度3.0%、壳聚糖浓度1.0%、CaCl2浓度5.5%、固化时间90min。固定化后的酶重复使用4次后,相对酶活力仍保留80%以上,固化效果较好。同时,固定化酶最适反应温度和热稳定性提高,反应pH向碱性偏移,酶学特性得到了改善。
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| 关 键 词： | 海藻酸钠 壳聚糖 菠萝茎蛋白酶 固定化 |
Study on sodium alginate-chitosan immobilization of stem bromelain |
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| GAO Shang-xin,ZHOU Bing-ru,ZHOU Jin-si,LI Yan-fei,ZHAO Li-chao.Study on sodium alginate-chitosan immobilization of stem bromelain[J].Science and Technology of Food Industry,2011(5):209-211. |
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| Authors: | GAO Shang-xin ZHOU Bing-ru ZHOU Jin-si LI Yan-fei ZHAO Li-chao |
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| Affiliation: | GAO Shang-xin,ZHOU Bing-ru,ZHOU Jin-si,LI Yan-fei,ZHAO Li-chao (College of Food Science,South China Agricultural University,Guangzhou 510000,China) |
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| Abstract: | Sodium alginate and chitosan materials were fixed to the stem bromelain,and the immobilization conditions and characteristics of immobilized stem bromelain were studied.Through single factor and orthogonal experiment,the best conditions for immobilized stem bromelain were determined as follow:enzyme dosage of 0.4mg/g,3.0% sodium alginate concentration,chitosan concentration of 1.0%,CaCl2 concentration of 5.5%,immobilized time of 90min.The relative activity of immobilized stem bromelain after repeated use fo... |
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| Keywords: | sodium alginate chitosan stem bromelain immobilization |
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