|
|||||
|
|
| 肠溶包衣乳糖酶纳米微囊的制备及稳定性研究 | |
| 引用本文： | 贺红军,孔保华,夏娟,姜竹茂. 肠溶包衣乳糖酶纳米微囊的制备及稳定性研究[J]. 食品工业科技, 2011, 0(5): 323-327 |
| 作者姓名： | 贺红军 孔保华 夏娟 姜竹茂 |
| 作者单位： | 1. 东北农业大学食品学院,黑龙江哈尔滨,150030;烟台大学化学生物理工学院,山东烟台,264005 2. 东北农业大学食品学院,黑龙江哈尔滨,150030 3. 烟台大学化学生物理工学院,山东烟台,264005 |
| 基金项目： | 国家高技术研究发展计划863项目，黑龙江省教委海外学人项目 |
| 摘 要： | 研制一种在胃和肠液中对乳糖酶具有保护作用的口服制剂。采用复乳法制备了肠溶包衣乳糖酶纳米微囊,对其理化性质进行了评价;研究了制备过程中影响乳糖酶活性的主要因素,并采取了一定的保护策略;对微囊化的乳糖酶在人工胃液和人工肠液中的稳定性进行了考察。结果表明,超声强度越大,活性损失越多;在二氯甲烷中引入乙酸乙酯(体积比为1∶1),复乳化采用高压均质,有利于保护乳糖酶的活性。在内水相添加牛血清白蛋白、聚乙烯醇和聚乙二醇400等保护剂,可有效提高乳糖酶的活性。制备的肠溶包衣乳糖酶纳米微囊在人工胃液中2h乳糖酶剩余活性为88.97%,在含胰酶的人工肠液中6h,其活性保留可达95%以上,有望成为乳糖酶口服制剂的有效剂型。 |
| 关 键 词： | 乳糖不耐受症 肠溶包衣 乳糖酶 纳米微囊 稳定性 |
Preparation and stability of enteric-coated nanocapsules loaded with lactase |
|
| HE Hong-jun,KONG Bao-hua,XIA Juan,JIANG Zhu-mao. Preparation and stability of enteric-coated nanocapsules loaded with lactase[J]. Science and Technology of Food Industry, 2011, 0(5): 323-327 | |
| Authors: | HE Hong-jun KONG Bao-hua XIA Juan JIANG Zhu-mao |
| Affiliation: | HE Hong-jun1,2,KONG Bao-hua1,XIA Juan2,JIANG Zhu-mao2 (1.College of Food Science,Northeast Agricultural University,Harbin 150030,China,2.Chemistry and Biology College,Yantai University,Yantai 264005,China) |
| Abstract: | An oral preparation which could protect lactase from inactivation in both the stomach and intestine was prepared.Enteric-coated nanocapsules loaded with lactase were prepared by double emulsion method and physicochemically characterized.The influence of main process parameters on the lactase stability loaded in nanocapsules was investigated,and some strategic approaches were taken for protection.The lactase stability loaded in nanocapsules in the simulated gastric juice and intestinal juice were also evalua... |
| Keywords: | lactose intolerance enteric-coated lactase nanocapsules stability |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |