Identification of a structural gene encoding a metallothionein-like domain that includes a putative regulator protein for Streptomyces protease gene expression |
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| Authors: | S Taguchi T Ogawa T Endo H Momose |
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| Affiliation: | Department of Biological Science and Technology, Science University of Tokyo, Chiba, Japan. staguchi@rs.noda.sut.ac.jp |
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| Abstract: | An open reading frame (termed ORF-PR) encoding a metallothionein-like domain-including protein was found upstream of a previously identified Streptomyces chymotrypsin-type protease gene (sam-P20). Promoter and terminator activities of ORF-PR were detected using the promoterless Streptomyces tyrosinase gene as a reporter gene and expression of ORF-PR was supposed to occur before that of sam-P20 gene. Frameshift mutation analysis showed that the ORF-PR product might act as a repressive regulator of the sam-P20 gene. |
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