| 响应面法优化微波提取酿酒酵母中谷胱甘肽条件的研究 |
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| 引用本文： | 俞志敏,栾静,郭继强,陈莉,汤佳鑫,张天雪,赵长新.响应面法优化微波提取酿酒酵母中谷胱甘肽条件的研究[J].大连工业大学学报,2008,27(3). |
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| 作者姓名： | 俞志敏 栾静 郭继强 陈莉 汤佳鑫 张天雪 赵长新 |
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| 摘 要： | 利用响应分析法考察了微波处理时间、提取时间和水料比3个因素对微波提取酿酒酵母胞内谷胱甘肽提取率的影响。研究发现,谷胱甘肽的最佳提取条件为:微波处理20.96 s、提取时间为13.25 min,加水量与干细胞的比为22.20 mL/g,谷胱甘肽的提取率为13.06 mg/g,比单因素试验最高的提取率高8.3%,且与预期值相符。
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| 关 键 词： | 谷胱甘肽 微波提取 酿酒酵母 回应面分析 |
Extraction of glutathione from Saccharomyces cerevisiae by microwave treating |
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| YU Zhi-min,LUAN Jing,GUO Ji-qiano,OHEN Li,TANG Jia-xin,ZHANG Tian-xue,ZHAO Chang-xin.Extraction of glutathione from Saccharomyces cerevisiae by microwave treating[J].Journal of Dalian Dalian Polytechnic University,2008,27(3). |
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| Authors: | YU Zhi-min LUAN Jing GUO Ji-qiano OHEN Li TANG Jia-xin ZHANG Tian-xue ZHAO Chang-xin |
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| Abstract: | The influence of microwave treating time,extraction time and ratio of water to material on the extraction of GSH from Saccharomyces cerevisiae was evaluated.The results showed that the optimum conditions of GSH extraction are: microwave treating time 20.96 s,extraction time 13.25 min,ratio of water to material 22.20 mL/g.The best extraction rate is 13.06 mg/g. |
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| Keywords: | glutathione microwave extraction Saccharomyces cerevisiae response surface analysis |
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