Antioxidant activity of enzymatic extracts from a brown seaweed Ecklonia cava by electron spin resonance spectrometry and comet assay |
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| Authors: | Soo-Jin Heo Pyo-Jam Park Eun-Ju Park Se-Kwon Kim You-Jin Jeon |
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| Affiliation: | (1) Faculty of Applied Marine Science, Cheju National University, Jeju, 690-756, South Korea;(2) Department of Biotechnology, Konkuk University, Chungju, 380-701, South Korea;(3) Department of Chemistry, Pukyong National University, Pusan, 608-737, South Korea;(4) Division of Life Sciences, Kyungnam University, Masan, 631-701, South Korea |
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| Abstract: | The potential antioxidant activity of enzymatic extracts from Ecklonia cava was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical scavenging using a electron spin resonance (ESR) spectrophotometer compared with ESR signal intensity and inhibitory effect of DNA damage on comet assay. E. cava was enzymatically hydrolyzed with five food industrial carbohydrases (AMG, Celluclast, Termamyl, Ultraflo and Viscozyme) from Novozyme Nordisk. All the tested extracts exhibited strong radical scavenging activities and the values were dose-dependent. The DPPH and hydroxyl radical scavenging activities of the enzymatic extracts were compatible with the vitamin C, which the alkyl radical scavenging activity was even higher than that of vitamin C. Also in the present study, the enzymatic extracts showed strong inhibitory effect against DNA damage in a dose dependent manner. These results indicated that the E. cava might be valuable natural antioxidative source. |
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| Keywords: | Ecklonia cava Enzymatic extract Antioxidant Electron spin resonance Radical scavenging activity DNA damage |
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