Tocopherol distribution and molecular species of triacylglycerols in soybean embryonic axes |
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Authors: | Hiromi Yoshida Yuka Tomiyama Saki Kanrei Yoshiyuki Mizushina |
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Affiliation: | (1) Department of Nutritional Science, Kobe Gakuin University, Nishi-ku, 651-2180 Kobe, Hyogo, Japan;(2) Life Science Center, Kobe Gakuin University, Nishi-ku, 651-2180 Kobe, Hyogo, Japan |
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Abstract: | Soybean embryonic axes were separated from other tissues, i.e., the cotyledons and seed coat. The molecular species and FA
distribution of TAG isolated from total lipids in the embryonic axes were analyzed by a combination of argentation-TLC and
GC, and were investigated in relation to their tocopherol distribution, which was determined by HPLC. The dominant components
were γ-tocopherols, with much smaller amounts of α-, β-, and δ-tocopherols. A modified argentation-TLC procedure, developed
to optimize the separation of the complex mixture of total TAG, provided 16 different groups of TAG, based on both the degree
of unsaturation and the total acyl-chain length of FA groups. With a few exceptions, the major TAG components were S2D (6.8–10.3%), SMD (6.9–11.2%), SD2 (7.2–9.8%), SMT (3.2–7.4%), SDT (11.5–19.5%), D3 (3.5–8.3%), MDT (4.5–7.7%), D2T (11.1–20.6%), and DT2 (8.2–15.7%) (where S denotes saturated FA, M denotes monoenes, D denotes dienes, and T denotes trienes). These results indicate
that there were significant differences (P<0.05) not only in tocopherol distribution but also in the molecular species of TAG among the four cultivars. Therefore, these
tissues should be made available as raw materials for soybean-germ oil or soy milk, based on the differences in the distributions
of tocopherol homologs and the molecular species of TAG within the embryonic axes. |
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Keywords: | AgNO3-TLC embryonic axis molecular species soybeans triacylglycerols vitamin E homologs |
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