响应面法优化油菜蜂花粉核苷类成分提取工艺

目的：考察油菜蜂花粉核苷类成分的提取条件，确定最佳提取工艺。方法：先通过单因素试验获得油菜蜂花粉核苷类成分提取时间、提取温度及料液比的优化范围，再利用Box-Behnken试验设计原理和3因素3水平的响应面分析法优化蜂花粉核苷类成分的提取工艺。结果：3因素的优化范围分别为40～60min、45～65℃、1:60～1:80(g/mL)。结论：最终优化条件为提取时间53min、温度60℃、料液比1:70(g/mL)，该条件下，油菜蜂花粉中核苷类成分总提取量为(705.2±0.006)mg/100g。

Optimization of Nucleosides Extraction from Rape Bee Pollen Using Response Surface Methodology

Nucleosides extraction from rape bee pollen was optimized using response surface methodology.The appropriate ranges of extraction time,extraction temperature and material-to-liquid ratio were obtained by one-factor-at-a-time experiments for subsequent optimization using response surface analysis based on a three-variable,three-level central composite design.The optimal ranges of extraction time,extraction temperature and material-to-liquid ratio were 40-60 min,45-65 ℃ and 1:60-1:80(g/mL),respectively.The optimized extraction conditions were 53 min,60 ℃,and 1:70(g/mL).Under these conditions,the extraction rate of nucleosides from rape bee pollen was(705.2 ±0.006) mg/100 g.