| 重吸附法回收利用纤维素酶的工艺优化 |
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| 引用本文： | 李强,张名佳,苏荣欣,齐崴,何志敏.重吸附法回收利用纤维素酶的工艺优化[J].化学工程,2010,38(2). |
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| 作者姓名： | 李强 张名佳 苏荣欣 齐崴 何志敏 |
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| 作者单位： | 天津大学,化工学院,化学工程国家重点联合实验室,天津,300072 |
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| 基金项目： | 国家自然科学基金资助项目(20976130);;国家科技支撑计划(2007BAD42B02);;国际科技合作计划(2006DA62400) |
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| 摘 要： | 以玉米芯为底物,分别考察了Tween 80质量分数、加酶量和底物质量分数对纤维素酶解过程中纤维素酶回收利用的影响规律,结果表明,酶回收率随Tween 80质量分数和加酶量的增加呈先增大再减小的趋势。此外,酶回收率随底物质量分数的增加而逐渐增加,确定重吸附法回收纤维素酶的最优工艺条件:Tween 80质量分数为0.23%;加酶量(以葡聚糖计)为40 FPU/g;底物质量分数为3.6%,在最优条件下,纤维素酶回收率可达83.0%。该研究为木质纤维素生物炼制过程中降低纤维素酶成本提供了有效的解决方法。
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| 关 键 词： | 纤维素酶 回收 玉米芯 重吸附 Tween80 |
Process optimization of cellulase re-adsorption for reutilization |
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| LI Qiang,ZHANG Ming-jia,SU Rong-xin,QI Wei,HE Zhi-min.Process optimization of cellulase re-adsorption for reutilization[J].Chemical Engineering,2010,38(2). |
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| Authors: | LI Qiang ZHANG Ming-jia SU Rong-xin QI Wei HE Zhi-min |
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| Affiliation: | State Key Laboratory of Chemical Engineering;School of Chemical Engineering and Technology;Tianjin University;Tianjin 300072;China |
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| Abstract: | The effect of Tween 80 mass fraction,enzyme loading and substrate mass fraction on the re-adsorption of cellulases which hydrolyzed the corncob was studied.The result shows that as mass fraction of Tween 80 and enzyme loading increase,the cellulase recovery first increases and then decreases gradually.The cellulase recovery increases gradually with the increasing of substrate mass fraction.The optimum process conditions are as follows: mass fraction of Tween 80 is 0.23%,enzyme loading(based on glucan) is 40... |
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| Keywords: | cellulase recovery corncob re-adsorption Tween 80 |
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