Production of FAME from acid oil model using immobilized <Emphasis Type="Italic">Candida antarctica</Emphasis> lipase |
| |
Authors: | Email author" target="_blank">Yomi?WatanabeEmail author Praphan?Pinsirodom Toshihiro?Nagao Takashi?Kobayashi Yutaka?Nishida Yoshiaki?Takagi Yuji?Shimada |
| |
Affiliation: | (1) Faculty of Agricultural Industry, King Mongkut's Institute of Technology, Landkrabang, 10520 Bangkok, Thailand;(2) The Nisshin OilliO Group, Ltd., 235-8558 Yokohama, Japan;(3) Osaka Municipal Technical Research Institute, 1-6-50 Morinomiya, Joto-ku, 536-8553 Osaka, Japan |
| |
Abstract: | Acid oil is a by-product in the neutralization step of vegetable oil refining and is an alternative source of biodiesel fuel.
A model substrate of acid oil, which is composed of TAG and FFA, was used in experiments on the conversion to FAME by immobilized
Candida antarctica lipase. FFA in the mixture of TAG/FFA were efficiently esterified with methanol (MeOH), but the water generated by the esterification
significantly inhibited methanolysis of TAG. We thus attempted to convert a mixture of TAG/FFA to FAME by a two-step process
comprising methyl esterification of FFA and methanolysis of TAG by immobilized C. antarctica lipase. The first reaction was conducted at 30°C in a mixture of TAG/FFA (1∶1, wt/wt) and 10 wt% MeOH using 0.5 wt% immobilized
lipase, resulting in efficient esterification of FFA. The reaction mixture after 24 h was composed of 49.1 wt% TAG, 1.3 wt%
FFA, 49.1 wt% FAME, and negligible amounts of DAG and MAG (<0.5 wt%). The reaction mixture was then dehydrated and used as
a substrate for the second reaction, which was conducted at 30°C in a solution of the dehydrated mixture and 5.5 wt% MeOH
using 6 wt% immobilized lipase. The activity of the lipase increased gradually when the reaction was repeated by transferring
the enzyme to a fresh substrate mixture. The activity reached a maximum after 6 cycles, and the content of FAME achieved was
>98.5 wt% after a 24-h reaction. The immobilized lipase was very stable in the first-and second-step reactions and could be
used for >100 d without significant loss of activity. |
| |
Keywords: | Acid oil biodiesel fuel Candida antarctica FAME immobilized enzyme lipase methanolysis methyl esterification |
本文献已被 SpringerLink 等数据库收录! |
|