A modified Langmuir model for the prediction of the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents |
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| Affiliation: | 1. China University of Mining and Technology, Xuzhou 221116, Jiangsu, China;2. Western University, London, Ontario N6A 5B9, Canada;1. Department of Chemical & Biochemical Engineering, The University of Western Ontario, 1151 Richmond St., London, Ontario N6A 3K7, Canada;2. Department of Biochemical Engineering, AVT – Aachener Verfahrenstechnik, RWTH Aachen University, Worringer Weg 1, 52074 Aachen, Germany;1. Department of Pathology, Forensic Medicine and Cytology, Clinical Hospital Center Split, Croatia;2. Department of Hematology, Clinical Hospital Center Split, Croatia;3. Department of Pathology, University Hospital Mostar, Bosnia and Herzegovina;1. Department of Chemical and Biochemical Engineering, Western University, 1151 Richmond Street, London, Ontario N6A 3K7, Canada;2. Dipartimento di Ingegeria Chimica, dei Materiali e della Produzione Industriali, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy;1. RWTH Aachen University, Aachener Verfahrenstechnik, Aachen, Germany;2. The University of Western Ontario, Department of Chemical and Biochemical Engineering, London, Ontario, Canada |
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| Abstract: | An empirical model modified from the Langmuir isotherm model to account for the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents has been proposed and tested against experimental and literature data. The equilibrium isotherms for BSA adsorption onto a polystyrenic anion exchanger, Diaion HPA25, were established for five different NaCl concentrations at 25°C, pH 7.0. The apparent Langmuir parameters in the new model (qm′ and Kd′), which replace the Langmuir parameters (qm and Kd) in the original Langmuir model, were determined by non-linear curve fitting. The proposed model has been shown to be applicable to various protein/adsorbent systems. |
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