红球菌H-412生长细胞脱除正十六烷中的有机硫

利用实验室筛选的能够通过“4S”途径进行脱硫代谢的红平红球菌H-412(Rhodococcus sp.H-412)作为生物脱硫催化剂,二苯并噻吩(DBT)为含硫模型化合物,考察了红球菌H-412生长细胞脱除正十六烷中有机硫的脱硫性能.在油相体积分数20％的反应体系中,相同浓度的生长细胞比休止细胞的脱硫效率高.利用红球菌H-412生长细胞在油相分率20％的情况下,初始发酵液菌体浓度为0.048 g/L,初始DBT浓度为0.25 mmol/L的条件下分批培养的脱硫效率较高,达到了57％.红球菌H-412生长细胞在油相体积分率分别为0,5％,10％和20％条件下进行脱硫,DBT的消耗量及二羟基联苯(2-HBP)的生成量随油相体积增加而增大,20％条件下均达最大值,脱硫效率最高.细胞生长量随油相体积增大而减少,无油存在的条件下,细胞生长量最大.

Biodesulfurization of Organic Sulfur in n-Hexadecane by Rhodococcus sp.H-412 Growing Cells

The Rhodococcus sp.H-412 screened in our lab,which could desulfurize via 4S pathway,was selected as biocatalyst to study the desulfurization performance by its growing cells,dibenzothiophene（DBT） and n-hexadecane were used for model organic-sulfur compound and model oil,respectively.The desulfurization efficiency（XDBS） of growing cells is higher than that of resting cells in the same biomass concentration when the oil phase was 20%（v/v）.When initial biomass concentration was 0.048 g/L and DBT concentration was 0.25 mmol/L,the higher XDBS was reached 57% under 20%（v/v） of hexadecane.The desulfurization by growing cells was carried out under the condition of 0%,5%,10%,20%（v/v） of hexadecane,DBT consumption and 2-HBP formation increased with the increasing of oil phase,and the highest XDBS was reached when the hexadecane was 20%.Biomass growth reduced with the increasing of oil volume,under oil-free condition,the biomass reached highest value.