口服ALA在皮肤及肿瘤中的代谢

目的 :探求ALA口服在皮肤及皮肤肿瘤中的代谢 ,寻求皮肤肿瘤光敏诊断最佳的ALA口服剂量及时间。方法 :口服不同剂量的ALA(2 0mg/kg、4 0mg/kg、6 0mg/kg、80mg/kg、10 0mg/kg) ,不同时间 (1小时、2小时、4小时、6小时、8小时、10小时、12小时、2 4小时、4 8小时 )以激光诱导荧光OMA检测系统测定肿瘤及左股部皮肤荧光 ,并经数据处理 ,寻求皮肤肿瘤与正常皮肤荧光相对值相差最大的合适的ALA口服剂量及诊断时间。结果 :脉宽 2 0或 30A皮肤肿瘤荧光值总和与组织荧光值总和之比除以正常皮肤荧光值与组织荧光值总和之比在口服ALA6 0mg/kg ,服ALA后 6 - 8小时时比值最大。结论 :从以上数据分析口服ALA作皮肤肿瘤OMA荧光光谱分析剂量以 6 0mg/kg ,服ALA后 6 - 8小时作荧光光谱分析较合适 ,计算相对值在峰值宽度 2 0 - 30A面积均可。

Metabolize of ALA Take Orally on Skin and Tumor

Objective: Search metabolize of ALA take orally on skin and tumor,seek first-rank dose and diagnostic time of ALA take orally.Methods:Different dose of ALA take orally(20mg/kg、40mg/kg、60mg/kg、80mg/kg、100mg/kg),different time after ALA take orally(1h、2h、4h、6h、8h、10h、12h、24h、48h),mensurate fluorescence of tumor and skin, when irradiated by laser. By analyzing the spectrum of fluorescence with optical mutichannel analyser(OMA), also the fluorescence intensity should be normalized. Result:The most ratio (Itumor/Inomal skin) was 60mg/kg, of ALA take orally and 6-8h after ALA take orally. Conclusion:Mensurate fluorescence of tumor of skin By analyzing the spectrum of fluorescence with optical mutichannel analyser(OMA), first-rank dose was 60mg/kg of ALA take orally, time after ALA take orally was6-8h.