| 响应面法优化菜籽皮可溶性膳食纤维提取工艺 |
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| 引用本文： | 王顺民,汤斌,余建斌,张浩.响应面法优化菜籽皮可溶性膳食纤维提取工艺[J].中国粮油学报,2011,26(9):98-103. |
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| 作者姓名： | 王顺民 汤斌 余建斌 张浩 |
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| 作者单位： | 1. 安徽工程大学生物与化学工程学院,芜湖,241000
2. 宁夏吴凯生物科技有限责任公司,银川75320 |
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| 基金项目： | 安徽省优秀青年人才基金(2010SQRL084ZD) |
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| 摘 要： | 为了探讨酶法和化学法结合提取菜籽皮中可溶性膳食纤维。采用纤维素酶和氢氧化钠提取菜籽皮中的可溶性膳食纤维,研究了酶添加量、酶解时间、碱解pH、碱解时间、碱解温度等因素对膳食纤维得率的影响。在单因素试验的基础上进行响应面试验设计,确定了酶-化学法制备菜籽皮膳食纤维的最佳工艺条件:纤维素酶加酶量为0.4%,酶解时间60 min,碱解pH 13,碱解温度70℃、碱解时间60 min,在此条件下菜籽可溶性膳食纤维得率为7.18%。因此,采用纤维素酶和氢氧化钠相结合的方法提取菜籽皮中的可溶性膳食纤维是切实可行的。
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| 关 键 词： | 菜籽皮 可溶性膳食纤维 酶-化学法 响应面分析 |
Response Surface Methodology as an Approach to Optimize the Extraction of Soluble Dietary Fiber from Rapeseed Shell |
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| Wang Shunmin,Tang Bin,Yu Jianbin,Zhang Hao.Response Surface Methodology as an Approach to Optimize the Extraction of Soluble Dietary Fiber from Rapeseed Shell[J].Journal of the Chinese Cereals and Oils Association,2011,26(9):98-103. |
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| Authors: | Wang Shunmin Tang Bin Yu Jianbin Zhang Hao |
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| Affiliation: | Wang Shunmin1 Tang Bin1 Yu Jianbin2 Zhang Hao1 (Biological and chemical engineering institute Anhui Polytechnic University1,Wuhu 241000) Ningxia Wukai Biological Science and Technology Ltd.2,Yinchuan 753202) |
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| Abstract: | The objective of this study was to gain better insight into the effects of the enzymatic and chemical method on the extraction of the soluble dietary fiber of rapeseed shell.In two methods,cellulose enzyme and NaOH were used to extract soluble dietary fiber of rapeseed shell.Effects of four crucial parameters(i.e.a cellulase dose,pH adjustment of water as extraction solvent,temperature and time) on extraction yield of soluble dietary fiber were investigated.On the basis of the single-factor experiment,the r... |
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| Keywords: | rapeseed shell soluble dietary fiber enzymatic and chemical method response surface analysis |
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