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| 中华稻蝗内生菌株SDLH抗菌肽的分离纯化 | |
| 引用本文： | 张迪迪,李敏,李婷,朱张洪基,宋强,黄登宇. 中华稻蝗内生菌株SDLH抗菌肽的分离纯化[J]. 食品研究与开发, 2011, 32(6): 8-10 |
| 作者姓名： | 张迪迪 李敏 李婷 朱张洪基 宋强 黄登宇 |
| 作者单位： | 1. 山西大学生物技术研究所,山西太原,030006 2. 山西省生物研究所,山西太原,030006 3. 山西中医学院基础部,山西太原,030024 4. 山西大学生物技术研究所，山西太原030006;山西省医药与生命科学研究院，山西太原030012 |
| 基金项目： | 山西省科技攻关项目(项目编号:20100321110) |
| 摘 要： | 采用PEG 6000沉淀法、DEAE-32阴离子层析和Sephadex G-50凝胶层析分离纯化SDLH抗菌肽,抑菌圈法测定抑菌活性,Bradford法测定蛋白含量,PAGE检测纯度。结果获得分子量为17.0 KD,电泳纯的抗菌肽。 |
| 关 键 词： | 中华稻蝗 抗菌肽 抑菌活性 分离纯化 |
Purification of Antibacterial Protein from Endophyte Strain in Oxya Chinensis |
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| ZHANG Di-di,LI Min,LI Ting,ZHU ZHANG Hong-ji,SONG Qiang,HUANG Deng-yu. Purification of Antibacterial Protein from Endophyte Strain in Oxya Chinensis[J]. Food Research and Developent, 2011, 32(6): 8-10 | |
| Authors: | ZHANG Di-di LI Min LI Ting ZHU ZHANG Hong-ji SONG Qiang HUANG Deng-yu |
| Affiliation: | ZHANG Di-di1,LI Min1,LI Ting2,ZHU ZHANG Hong-ji1,SONG Qiang3,HUANG Deng-yu1,4,(1.Shanxi University,Institute of Biotechnology,Taiyuan 030006,Shanxi,China,2.Institute of Shanxi Biology,3.Department of Basic Sciences,Shanxi College of Traditional Chinese Medicine,Taiyuan 030024,4.Institute of Shanxi Chinese Medicine and Life Sciences,Taiyuan 030012,China) |
| Abstract: | The Antibacterial protein is extracted and purified by the PEG 6 000 method and DEAE-32 chromatography and Sephadex G-50 chromatography,the Zone of inhinition method determinated the antibacterial activities.Bradford method determinated the contents of protein.The PAGE method detected the purity of protein.The result is that the purified protein obtained by electrophoresis has the molecular weight of 17.0 KD. |
| Keywords: | Oxya Chinensis antibacterial protein antibacterial activities purification |
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