高效液相色谱-荧光检测法测定蜂蜜中的果糖、葡萄糖和麦芽糖

建立反相离子对色谱法同时测定蜂蜜中果糖、葡萄糖、麦芽糖含量的方法。样品经对氨基苯甲酸衍生化后，采用ODS柱，以0.1mol/L磷酸盐缓冲液(20mmol/L四丁基硫酸氢铵，pH2.5):乙腈=95:5为流动相，荧光检测器检测，外标法定量。结果表明，果糖、葡萄糖、麦芽糖的检出限分别为210、96、240μg/kg，线性范围分别为130～140、52～98、154～98mg/kg，在其线性范围内相关系数r≥0.9988；该方法加标回收率在98.2%～101.2%之间，RSD在3.4%～5.4%之间。该方法灵敏、结果可靠。

Determination of Fructose, Glucose, and Maltose in Honey by HPLC-FLD

An ion-pair reversed-phase high performance liquid chromatographic method was developed to simultaneous determine fructose,glucose,and maltose in honey using p-aminobenzoic acid(p-AMBA) for pre-column derivatization.The chromatographic separation was achieved on an ODS column using a mobile phase made up of 0.1 mol/L phosphate buffer solution(20 mmol/L tetrabutylammoniumhydrogen sulfate,pH 2.5) and acetonitrile(95:5,V/V).The analytes were detected using a fluorescence detector and quantified by external sta...