| 新霉素ELISA检测方法的建立 |
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| 引用本文： | 刘沙洲,桑小雪,欧阳华学,雷绍荣,白林含. 新霉素ELISA检测方法的建立[J]. 食品科学, 2011, 32(14): 227-231. DOI: 10.7506/spkx1002-6630-201114048 |
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| 作者姓名： | 刘沙洲 桑小雪 欧阳华学 雷绍荣 白林含 |
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| 作者单位： | 1. 四川大学生命科学学院2.成都市食品药品检测中心3.四川省农业科学院分析测试中心 |
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| 基金项目： | 四川省公益性研究计划项目(2008NG004) |
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| 摘 要： | 目的：比较直接和间接竞争酶联免疫法(enzyme linked immunosorbent assay，ELISA)的优缺点，建立新霉素残留ELISA检测方法。方法：利用自制的新霉素多克隆抗体，采用直接竞争和间接竞争ELISA方法检测新霉素残留，并比较两种方法的优缺点。结果：新霉素抗血清和庆大霉素的交叉反应率为2.04%，和卡那霉素的交叉反应率为0.02%，和氨苄青霉素、红霉素、四环素的交叉反应率均小于0.01%。初步测试新霉素间接竞争ELISA法的准确性和回收率。板内误差小于4%，板间误差小于11%，回收率为135.5%～191.3%。直接竞争和间接竞争ELISA方法的检测极限分别为28.58ng/mL和51.74ng/mL，达到了国家对新霉素规定的500μg/kg MRL检测限。结论：建立了直接竞争和间接ELISA吸附检测方法，条件优化更成功的间接竞争ELISA可用于开发新霉素检测试剂盒。
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| 关 键 词： | 新霉素 多克隆抗体 竞争酶联免疫法(enzyme linked immunosorbent assay ELISA) 方法建立 |
Development of Enzyme-linked Immunosorbent Assay for Neomycin |
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| LIU Sha-zhou,,SANG Xiao-xue,OUYANG Hua-xue,LEI Shao-rong,BAI Lin-han. Development of Enzyme-linked Immunosorbent Assay for Neomycin[J]. Food Science, 2011, 32(14): 227-231. DOI: 10.7506/spkx1002-6630-201114048 |
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| Authors: | LIU Sha-zhou SANG Xiao-xue OUYANG Hua-xue LEI Shao-rong BAI Lin-han |
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| Affiliation: | (1. School of Life Sciences, Sichuan University, Chengdu 610065, China；2. Food and Drug Testing Center, Chengdu 610045,China；3. Analysis and Testing Center, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China) |
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| Abstract: | In this study,we describe the advantages and disadvantages of direct competitive enzyme-linked immunosorbent assay(dc-ELISA) and indirect competitive ELISA(idc-ELISA) and ELISA methods for the detection of neomycin.Anti-neomycin polyclonal antibodies were prepared and used to detect neomycin by dc-ELISA and idc-ELISA.The cross-reaction rates of prepared anti-neomycin polyclonal antibodies with gentamincin and kanamycin were 2.04% and 0.02%,respectively,and with ampicillin,erythromycin and tetracycline all l... |
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| Keywords: | neomycin polyclonal antibodies competitive enzyme-linked immunosorbent assay(ELISA) method establishment |
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