| 应用SELEX技术筛选沙门氏菌抗原的适配子 |
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| 引用本文： | 郎春燕,江树勋,邵碧英,陈文炳,傅碧忠,缪婷玉,陈融斌,黄晓蓉. 应用SELEX技术筛选沙门氏菌抗原的适配子[J]. 食品科学, 2011, 32(13): 194. DOI: 10.7506/spkx1002-6630-201113042 |
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| 作者姓名： | 郎春燕 江树勋 邵碧英 陈文炳 傅碧忠 缪婷玉 陈融斌 黄晓蓉 |
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| 作者单位： | 福建出入境检验检疫局福建省检验检疫技术研究重点实验室;福建农林大学食品科学学院;集美大学水产学院; |
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| 基金项目： | 国家质检总局科技计划项目(2008IK164); 福建省科技攻关计划重点项目(2008Y0001) |
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| 摘 要： | 目的:应用指数富集配体系统进化(SELEX)技术筛选沙门氏菌抗原的高亲和性适配子。方法:首先合成一个全长78个核苷酸中间含35个随机序列的随机单链寡核苷酸序列(ssDNA)文库,再以环氧乙烷丙烯酸珠子作为筛选介质,利用生物素-抗地高辛碱性磷酸酶显色系统检测DNA适配子与沙门氏菌抗原的亲和力,以获得沙门氏菌抗原的高亲和性适配子。结果:随着筛选轮数的增加,DNA适配子与沙门氏菌抗原结合后显色,吸光度逐渐增加,初步获得了沙门氏菌抗原的高亲和性适配子。结论:实验所用的筛选流程是适当的,可以考虑推广用于类似靶目标的筛选。
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| 关 键 词： | 指数富集配体系统进化技术 沙门氏菌 抗原 适配子 |
Screening of High-affinity DNA Aptamers to Salmonella Antigen by SELEX Technique |
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| LANG Chun-yan,,JIANG Shu-xun,SHAO Bi-ying,CHEN Wen-bing,FU Bi-zhong,MIAO Ting-yu,CHEN Rong-bin,HUANG Xiao-rong. Screening of High-affinity DNA Aptamers to Salmonella Antigen by SELEX Technique[J]. Food Science, 2011, 32(13): 194. DOI: 10.7506/spkx1002-6630-201113042 |
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| Authors: | LANG Chun-yan JIANG Shu-xun SHAO Bi-ying CHEN Wen-bing FU Bi-zhong MIAO Ting-yu CHEN Rong-bin HUANG Xiao-rong |
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| Affiliation: | LANG Chun-yan1,2,JIANG Shu-xun1,SHAO Bi-ying1,CHEN Wen-bing1,FU Bi-zhong1,MIAO Ting-yu1,CHEN Rong-bin3,HUANG Xiao-rong1(1.Fujian Key Laboratory for Technology Research of Inspection and Quarantine,Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou 350001,China,2.College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,3.Fisheries College of Jimei University,Xiamen 361021,China) |
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| Abstract: | Objective:To screen high-affinity aptamers to Salmonella antigen by applying SELEX(system evolution of ligands by exponential enrichment) method and establish a rapid detection method for Salmonella in food.Methods:First,a single stranded DNA(ssDNA) random library with 78 nucleotides in length containing 35 random sequences was constructed.Subsequently,Eupergit C was used as the screening medium in the SELEX method.The binding of aptamers to Salmonella antigen was visualized by Biotin-Streptavidin-Anti-Digo... |
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| Keywords: | system evolution of ligands by exponential enrichment(SELEX) Salmonella antigen aptamer |
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