| 蚕蛹多糖脱蛋白方法研究 |
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| 引用本文： | 伍善广,赖泰君,孙建华,刘旭辉,童张法,廖丹葵. 蚕蛹多糖脱蛋白方法研究[J]. 食品科学, 2011, 32(14): 21-24. DOI: 10.7506/spkx1002-6630-201114005 |
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| 作者姓名： | 伍善广 赖泰君 孙建华 刘旭辉 童张法 廖丹葵 |
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| 作者单位： | 1. 广西大学化学化工学院2.柳州医学高等专科学校3.河池学院化学与生命科学系 |
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| 基金项目： | 广西自然科学基金项目(桂科自0728237); 广西壮族自治区教育厅科研项目(200607MS031); 广西大学基金项目(X071029) |
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| 摘 要： | 以蛋白去除率和多糖损失率为指标，比较Sevag法、三氯乙酸(trichloroacetic acid，TCA)法、木瓜蛋白酶法、木瓜蛋白酶-Sevag联用法和木瓜蛋白酶-TCA联用法脱除蚕蛹多糖蛋白的效果。结果显示：木瓜蛋白酶-TCA联用效果最好，其脱蛋白的最佳条件为木瓜蛋白酶用量[E]/[S] 2.0%、酶解温度50℃、酶解反应1.5h、采用TCA法除蛋白1次，此时蛋白去除率为97.98%，多糖损失率为18.76%；紫外光谱扫描定性实验显示脱蛋白的多糖在260～280nm处只有少量吸收，表明大多数蛋白已去除。木瓜蛋白酶-TCA联用法是一种有效的多糖脱蛋白方法。
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| 关 键 词： | 多糖 脱蛋白 蚕蛹 |
A Comparative Investigation into Deproteinization Methods for Silkworm Pupa Polysaccharides |
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| WU Shan-gang,,LAI Tai-jun,SUN Jian-hua,LIU Xu-hui,,TONG Zhang-fa,LIAO Dan-kui. A Comparative Investigation into Deproteinization Methods for Silkworm Pupa Polysaccharides[J]. Food Science, 2011, 32(14): 21-24. DOI: 10.7506/spkx1002-6630-201114005 |
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| Authors: | WU Shan-gang LAI Tai-jun SUN Jian-hua LIU Xu-hui TONG Zhang-fa LIAO Dan-kui |
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| Affiliation: | (1. College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China；2. Liuzhou Medical College, Liuzhou 545006, China；3. Department of Chemistry and Life Science, Hechi University, Yizhou 546300, China) |
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| Abstract: | Protein removal rate and polysaccharide loss rate were used to evaluate and compare the effectiveness of Savag, s method, trichloroacetic acid (TCA) method, papain method and papain-TCA (papain hydrolysis followed by TCA precipitation) method in deproteinizing silkworm pupa polysaccharides. Papain-TCA method was found to be the best method for the deproteinization of silkworm pupa polysaccharides, and the optimal operating conditions were 1.5 h hydrolysis with papain at an enzyme-to-substrate of 2.0% and 50 ℃ followed by once treatment with TCA. A protein removal rate of 97.98% and a polysaccharide loss rate of 18.76% were achieved under such conditions. Only one absorption peak was observed in the UV-visible scanning spectrum of deporteinized silkworm pupa polysaccharides, indicating the removal of almost all proteins. |
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| Keywords: | pupa polysaccharide protein removal |
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