| 鼠伤寒沙门氏菌特异性多克隆抗体的纯化方法 |
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| 引用本文： | 牛瑞江,赖卫华,熊齐荣,魏华,熊勇华. 鼠伤寒沙门氏菌特异性多克隆抗体的纯化方法[J]. 食品科学, 2011, 32(13): 151-155. DOI: 10.7506/spkx1002-6630-201113033 |
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| 作者姓名： | 牛瑞江 赖卫华 熊齐荣 魏华 熊勇华 |
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| 作者单位： | 南昌大学食品科学与技术国家重点实验室 |
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| 基金项目： | 科技部中小企业创新基金项目(10C26223601934) |
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| 摘 要： | 以福尔马林灭活的鼠伤寒沙门氏菌免疫雌性日本大耳兔，制备抗沙门氏菌的多克隆抗体。分别通过辛酸-硫酸铵沉淀法、磁珠偶联抗原吸附法纯化抗体，所制备的抗体效价为3.2×105，纯度较高，但交叉反应现象较严重。本实验以杂菌抗原反向吸附法纯化抗体，有效消除了抗体与杂菌的交叉反应，初步建立了一种高特异性多克隆抗体的纯化方法。
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| 关 键 词： | 关键词：鼠伤寒沙门氏菌 多克隆抗体 反向吸附 交叉反应 |
Purification of Specific Polyclonal Antibody against Salmonella typhimurium |
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| NIU Rui-jiang,LAI Wei-hua,XIONG Qi-rong,WEI Hua,XIONG Yong-hua. Purification of Specific Polyclonal Antibody against Salmonella typhimurium[J]. Food Science, 2011, 32(13): 151-155. DOI: 10.7506/spkx1002-6630-201113033 |
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| Authors: | NIU Rui-jiang LAI Wei-hua XIONG Qi-rong WEI Hua XIONG Yong-hua |
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| Affiliation: | State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China |
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| Abstract: | A highly specific anti-Salmonella typhimurium polyclonal antibody was prepared for immunological determination of Salmonella typhimurium. Anti-Salmonella typhimurium polyclonal antibody was generated in Japanese rabbits using formalin-inactivated Salmonella typhimurium as antigen and purified by octanoic acid-ammonium sulfate precipitation and magnetic bead-antigen coupling. The titer of the prepared highly pure antibody was 3.2 × 105; however, this antibody revealed serious cross-reaction with other bacteria. In this paper, the antibody was purified by miscellaneous bacterial antigens in a reverse adsorption mode, which could eliminate the cross reaction between the antibody and miscellaneous bacteria. Therefore, an effective purification method to obtain specific polyclonal antibody was established. |
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| Keywords: | Salmonella typhimurium polyclonal antibody reverse adsorption cross reactivity |
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