| 耐氨米根霉菌株ST50-2产L-乳酸发酵条件研究 |
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| 引用本文： | 唐莹,姜绍通,吴学凤,李兴江,罗水忠,郑志.耐氨米根霉菌株ST50-2产L-乳酸发酵条件研究[J].食品科学,2011,32(13):145-150. |
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| 作者姓名： | 唐莹 姜绍通 吴学凤 李兴江 罗水忠 郑志 |
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| 作者单位： | 合肥工业大学生物与食品工程学院 |
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| 基金项目： | 国家“863”计划重点项目(2007AA10Z361); 安徽省农产品精深加工重点实验室项目(2009AKSY0102); 安徽省自然科学基金项目(090411015) |
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| 摘 要： | 为研究耐氨米根霉菌株ST50-2产L-乳酸的发酵特性，采用氨水中和剂，进行7L反应器发酵实验。与ST50-2的出发菌株AS3.819相比，发酵周期由原来的72h缩短为56h，L-乳酸产量提高12.30%，乙醇含量降低22.22%，乳酸脱氢酶(LDH)比活力提高83.09%，乙醇脱氢酶(ADH)比活力降低16.06%，对还原糖的利用速率及生物量的积累均高于出发菌株。7L反应器发酵实验表明：CaCO3影响菌体的成球；搅拌转速、氨水体积分数影响菌体的生长、成球大小及L-乳酸产量；pH值影响L-乳酸产量及发酵周期。其优化结果为：在搅拌转速400r/min，中和剂氨水体积分数为15%，pH值控制在6.0时，ST50-2菌球直径为1.0～1.5mm，发酵周期为56h，L-乳酸产量达93.32g/L。
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| 关 键 词： | 耐氨米根霉 L-乳酸 乳酸脱氢酶 乙醇脱氢酶 |
Optimization of Fermentation Conditions for L-Lactic Acid Production by Ammonia-tolerant Rhizopus oryzae Mutant ST50-2 |
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| TANG Ying,JIANG Shao-tong,WU Xue-feng,LI Xing-jiang,LUO Shui-zhong,ZHENG Zhi.Optimization of Fermentation Conditions for L-Lactic Acid Production by Ammonia-tolerant Rhizopus oryzae Mutant ST50-2[J].Food Science,2011,32(13):145-150. |
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| Authors: | TANG Ying JIANG Shao-tong WU Xue-feng LI Xing-jiang LUO Shui-zhong ZHENG Zhi |
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| Affiliation: | School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China |
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| Abstract: | Fermentation experiments in a 7 L bioreactor were carried out to study fermentation characteristics of ammonia-tolerant Rhizopus oryzae mutant ST50-2 and L-lactic acid production using aqueous ammonia as the neutralizer.Compared with its parental strain Rhizopus oryzae AS3.189,the mutant strain resulted in a reduction in fermentation period from 72 h to 56 h,in alcohol content by 22.22% and in ADH specific activity by 16.06% and an increase in L-lactic acid production by 12.30%,in LDH specific activity by 8... |
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| Keywords: | ammonia-tolerant Rhizopus oryzae L-lactic acid lactate dehydrogenase(LDH) alcohol dehydrogenase(ADH) |
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