| 基于流式分析技术的奶粉中金黄色葡萄球菌活菌快速定量检测方法研究 |
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| 引用本文： | 王斌,隋志伟,刘思渊,王晶,傅博强,王义. 基于流式分析技术的奶粉中金黄色葡萄球菌活菌快速定量检测方法研究[J]. 计量学报, 2021, 42(2): 250-258. DOI: 10.3969/j.issn.1000-1158.2021.02.19 |
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| 作者姓名： | 王斌 隋志伟 刘思渊 王晶 傅博强 王义 |
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| 作者单位： | 吉林农业大学生命科学学院,吉林长春130118;中国计量科学研究院前沿计量科学中心,北京100029;中国计量科学研究院前沿计量科学中心,北京100029;吉林农业大学生命科学学院,吉林长春130118 |
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| 基金项目： | 公益性科研院所基本科研业务费专项;国家重点研发计划 |
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| 摘 要： | 建立了一种奶粉中金黄色葡萄球菌活菌快速定量检测新方法.以异硫氰酸荧光素偶联的金黄色葡萄球菌多克隆抗体特异性标记金黄色葡萄球菌,再通过碘化丙啶来区分死活菌,建立流式分析法,并应用于奶粉样品中金黄色葡萄球菌活菌定量检测.结果表明,该流式分析法特异性好;当人工污染奶粉样品中金黄色葡萄球菌浓度在2.72 × 103～2.80 ...
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| 关 键 词： | 计量学 金黄色葡萄球菌活菌 奶粉 流式分析法 快速检测 |
| 收稿时间： | 2019-02-22 |
Rapid and Quantitative Detection of Viable Staphylococcus aureus in Milk Powder Based on Flow Cytometry Technology |
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| WANG Bin,SUI Zhi-wei,LIU Si-yuan,WANG Jing,FU Bo-qiang,WANG Yi. Rapid and Quantitative Detection of Viable Staphylococcus aureus in Milk Powder Based on Flow Cytometry Technology[J]. Acta Metrologica Sinica, 2021, 42(2): 250-258. DOI: 10.3969/j.issn.1000-1158.2021.02.19 |
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| Authors: | WANG Bin SUI Zhi-wei LIU Si-yuan WANG Jing FU Bo-qiang WANG Yi |
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| Affiliation: | 1. College of Life Science, Jilin Agricultural University, Changchun,Jilin 130118, China
2. Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China |
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| Abstract: | A flow cytometry(FCM)-based method for rapid and quantitative detection of viable Staphylococcus aureus in milk powder was developed.Staphylococcus aureus was labelled with Fluorescein isothiocyanate(FITC)by reacting with the anti-Staphylococcus aureus polyclonal antibody(FITC),then dead and viable bacteria were distinguished by membrane-impermeable dyes Propidium Iodid(PI).The FCM-based method was established and applied to the quantitative detection for Staphylococcus aureus in milk powder samples.The results showed that the FCM-based method exhibited high specificity towards Staphylococcus aureus.When the concentration of Staphylococcus aureus in artificially contaminated milk powder samples ranged from 2.72×103~2.80×108 CFU/mL,the FCM-based method and plate-based method yielded the same result,and the detection limit of the method is 2.72×103 CFU/mL,and total time is 35 min.The method is rapid and efficient,so it has a great application prospect in rapid screening and monitoring of foodborne pathogens. |
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| Keywords: | metrology viable Staphylococcus aureus milk powder flow cytometry rapid detection |
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