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Isolation and characterization of biosurfactant producing microorganisms from petroleum contaminated soil samples for EOR and bioremediation
Authors:Prasenjit Talukdar  Chinmoyee Sharma  Amolina Doley  Kimti Baruah  Ananya Borah  Payal Agarwal
Affiliation:1. Department of Petroleum Engineering, Dibrugarh University, Dibrugarh, Assam, India;2. Department of Petroleum Engineering, Indian School of Mines, Dhanbad, Jharkhand, India;3. Department of Health, Safety and Environment Engineering, University of Petroleum and Energy Studies, Dehradun, Uttarakhand, India
Abstract:Using of biosurfactant producing microorganisms for pipeline cleaning, bioremediation, enhanced oil recovery, etc. is a much soughed approach of petroleum industry. Although, many alternatives are available to serve these purposes, being ecofriendly and economic, microbial application is considered superior than most of the alternatives. This paper presents a comprehensive study on effectiveness of bacterial strain isolated from oil contaminated soil sample of Lakwa oilfield for required purposes. Its temperature sustainability and surfactant production capability was studied. Further characteristics of the produced biosurfactant were assessed through Emulsification Index measurement, Fourier Transform Infrared Spectra, Drop Collapse Test, Contact Angle and Viscosity measurement.
Keywords:biosurfactant  contact angle  drop collapse  emulsification index  fourier transform infrared spectra  pseudomonas species  thermal stability  viscosity reduction
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