Cyclopropaneoctanoic Acid 2‐Hexyl Upregulates the Expression of Genes Responsible for Lipid Synthesis and Release in Human Hepatic HepG2 Cells |
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| Authors: | Aleksandra Czumaj Adriana Mika Micha? Chmielewski Tomasz Sledzinski |
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| Affiliation: | 1. Department of Pharmaceutical Biochemistry, Medical University of Gdansk, 1 Debinki Street, Gdansk, Poland;2. Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, Gdansk, Poland;3. Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdansk, Debinki 7, Gdansk, Poland |
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| Abstract: | Recently we have found cyclopropaneoctanoic acid 2‐hexyl (CPOA2H) in humans and demonstrated its elevated levels in patients with metabolic diseases associated with hypertriglyceridemia. However, it is still unclear whether CPOA2H may influence lipid metabolism in lipogenic tissues. To verify this, HepG2 hepatocytes and 3T3‐L1 adipocytes were cultured with various concentrations of CPOA2H, and then the expressions of genes associated with lipid metabolism were determined. Incubation with CPOA2H at concentrations found in patients with metabolic diseases enhanced the expression of hepatocyte genes associated with lipid synthesis and release, in particular, the fatty acid synthase gene (nearly 20‐fold increase in the mRNA level). In contrast, incubation with CPOA2H caused the downregulation of most adipocyte genes associated with lipid synthesis, whereas the level of leptin mRNA was increased. These findings suggest that CPOA2H may contribute to hypertriglyceridemia in patients with metabolic diseases, upregulating the expression of hepatocyte genes responsible for lipid synthesis and release. |
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| Keywords: | Adipocyte Cyclopropane fatty acid Fatty acid synthase Hepatocyte Hypertriglyceridemia |
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