菥蓂提取物体外抗氧化活性研究

研究了菥蓂提取物的体外抗氧化活性,筛选有效活性部位。通过对菥蓂水提物的有机溶剂萃取、D101大孔树脂分离纯化,制备得到8个部位样品。以抗坏血酸作为阳性对照,通过考查各部位还原Fe3+的能力、清除二苯基苦基苯肼自由基(DPPH.)和羟基自由基(.OH)的能力、抑制油脂氧化的能力,来评价菥蓂水提物不同部位的抗氧化能力。结果表明:菥蓂的各部位均具有一定的抗氧化能力,但抗氧化活性总体上弱于抗坏血酸和BHT。大孔树脂分离得到的30%乙醇洗脱部位(VII)和乙酸乙酯萃取部位(II)抗氧化能力明显强于菥蓂水提物,稍弱于抗坏血酸。得到结论为萃取、大孔树脂分离纯化等步骤能富集更多的抗氧化成分。

In Vitro Antioxidant Activity of Extracts of Thlaspi arvense Linn Herb

The crude extract of Thlaspi arvense herb was obtained by reflux of water,and then 8 fractions were prepared by different solvent extraction,D101 macroporous resin separation successively.Compared with the ascorbic acid,antioxidant activities of these fractions were evaluated in vitro including reducing Fe3+,scavenging DPPH· free radical and hydroxyl free radical,and inhibiting peroxide oxidation of peanut oil.All fractions were found to have antioxidant activities but weaker than ascorbic acid.Furthermore,the data generated showed that 30% ethanolic elution of n-butanol extract by macroporous resin(VII) and the ethyl acetate extract(II) had better antioxidant activity obviously than crude extract,but slightly weaker than ascorbic acid.It also explained that different solvent extraction,macroporous resin separation could enrich more antioxidant contents.