Aggregation arrestant pheromone of the German cockroach,Blattella germanica (L.) (Dictyoptera: Blattellidae): Isolation and structure elucidation of blattellastanoside-A and -B |
| |
Authors: | Masayuki Sakuma Hiroshi Fukami |
| |
Affiliation: | (1) Present address: Faculty of Science and Technology, Ritsumeikan University, 56-1 Toji-in, Kitaku, 603 Kyoto, Japan;(2) Pesticide Research Institute Faculty of Agriculture, Kyoto University, Kitashirakawa, Sakyoku, 606 Kyoto, Japan |
| |
Abstract: | The aggregation pheromone of the German cockroach,Blattella germanica, consists of attractant and arrestant, which can be detected by olfactometer and choice-chamber assay, respectively. Both were extracted from the frass-contaminated filter paper being used as a shelter. They were separated by solvent partition withn-butanol and water. The arrestant from then-butanol phase was purified by open column chromatography and then successive HPLC isolated two major arrestant components. Spectral evidence from SI-MS, HR-EI-MS, and NMR experiments with pulse techniques provided possible structures as 1-(6-chloro-4,5-epoxy-5-stigmast-3-yl)--d-glucopyranoside and 1-(6-chloro-5-hydroxy-5-stigmast-3-yl)--d-glu-copyranoside, denoted as blattellastanoside-A and blattellastanoside-B, respectively. They represented arrestant activity as median effective doses (ED50) at 0.044 (A) and 3.2 (B) nmol on 1.0 cm2 of Whatman No. 1 filter paper. |
| |
Keywords: | Dictyoptera Blattellidae Blattella germanica German cockroach cockroach arrestant orientation pheromone aggregation pheromone steroid blattellastanoside |
本文献已被 SpringerLink 等数据库收录! |
|