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Scanning electron microscopic imaging of palynological metaphors: A case study of family Poaceae taxa
Authors:Nidaa Harun  Shabnum Shaheen  Mushtaq Ahmad  Zaheer Abbas  Fozia Bibi  Fahim Arshad
Affiliation:1. Department of Botany, Faculty of Life Sciences, University of Okara, Okara, Pakistan;2. Department of Botany, Lahore College for Women University, Lahore, Pakistan;3. Department of Plant Sciences, Quaid i Azam University, Islamabad, Pakistan;4. Department of Botany, Division of Science and Technology, University of Education, Lahore, Pakistan;5. Department of Botany, Rawalpindi Women University, Rawalpindi, Pakistan
Abstract:This study highlighted the taxonomic utilization of palynological metaphors for selected members (53) of family Poaceae. Multiple microscopic technique light and scanning electrons had been employed for detailed analysis. Results reported monad pollen type in all studied 53 members, which showed its limited taxonomic value up to family level. In relation to shape of pollen both polar and equatorial views strikingly differed from each other. Like semi angular pollen observed in Dactyloctenium aegyptium (L.) Wild. whereas round-minutely irregular pollen in Lolium temulentum L. Polar and equatorial diameter also showed variation, that is, Desmostachya bipinnata (L.) Stapf. can be differentiated from Sorghum bicolor (L.) Moench on diameter variation basis. A pore characteristic does not show much qualitative variation; however pore sizes differ species to species. The most frequently scarbate sculpturing was observed in 28 species followed by verrucate. Hence it can be said that pollen shape, polar, and equatorial diameters, pore size, P/E ratio, pore sculpturing are of good taxonomic value and holds a significant position in identification and delimitation of Poaceae taxa.
Keywords:microscopic imaging  monad pollen  palynology  Poaceae  scarbate  verrucate
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