Ion mobility mass spectrometry in the omics era: Challenges and opportunities for metabolomics and lipidomics |
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Authors: | Giuseppe Paglia Andrew J Smith Giuseppe Astarita |
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Affiliation: | 1. School of Medicine and Surgery, University of Milano-Bicocca, Vedano al Lambro (MB), Italy;2. Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, District of Columbia, USA |
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Abstract: | Researchers worldwide are taking advantage of novel, commercially available, technologies, such as ion mobility mass spectrometry (IM-MS), for metabolomics and lipidomics applications in a variety of fields including life, biomedical, and food sciences. IM-MS provides three main technical advantages over traditional LC-MS workflows. Firstly, in addition to mass, IM-MS allows collision cross-section values to be measured for metabolites and lipids, a physicochemical identifier related to the chemical shape of an analyte that increases the confidence of identification. Second, IM-MS increases peak capacity and the signal-to-noise, improving fingerprinting as well as quantification, and better defining the spatial localization of metabolites and lipids in biological and food samples. Third, IM-MS can be coupled with various fragmentation modes, adding new tools to improve structural characterization and molecular annotation. Here, we review the state-of-the-art in IM-MS technologies and approaches utilized to support metabolomics and lipidomics applications and we assess the challenges and opportunities in this growing field. |
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Keywords: | CCS cIM-MS collision cross-section DTIM-MS FAIM-MS IM-MS lipids metabolites MS imaging MSI spatial lipidomics spatial metabolomics TIM-MS TWIM-MS |
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