Hydroxyapatite coating of cellulose sponges attracts bone-marrow-derived stem cells in rat subcutaneous tissue |
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Authors: | Miretta Tommila Anne Jokilammi Perttu Terho Timothy Wilson Risto Penttinen Erika Ekholm |
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Affiliation: | 1.Department of Medical Biochemistry and Genetics, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland;2.Department of Anesthesiology, Intensive Care, Emergency and Pain Medicine, Turku University Hospital, 20521 Turku, Finland;3.Cell Imaging Core, Turku Centre for Biotechnology, 20520 Turku, Finland;4.Turku Biomaterials Centre, University of Turku, 20520 Turku, Finland |
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Abstract: | The presence of bone-marrow-derived stem cells was investigated in a wound-healing model where subcutaneously implanted cellulose sponges were used to induce granulation tissue formation. When cellulose was coated with hydroxyapatite (HA), the sponges attracted circulating haemopoietic and mesenchymal progenitor cells more efficiently than uncoated cellulose. We hypothesized that the giant cells/macrophages of HA-coated sponges recognize HA as foreign material, phagocyte or hydrolyse it and release calcium ions, which are recognized by the calcium-sensing receptors (CaRs) expressed on many cells including haemopoietic progenitors. Our results showed, indeed, that the HA-coated sponges contained more CaR-positive cells than untreated sponges. The stem cells are, most probably, responsible for the richly vascularized granulation tissue formed in HA-coated sponges. This cell-guiding property of HA-coated cellulose might be useful in clinical situations involving impaired wound repair. |
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Keywords: | wound healing granulation tissue cellulose sponge hydroxyapatite coating stem cells calcium-sensing receptor |
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