| 邻联茴香胺-H_2O_2-辣根过氧化物酶伏安酶联免疫分析检测四种重要植物病毒 |
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| 引用本文： | 焦奎,韦璐,张书圣,刘澄凡,张成良,张作芳,刘靖宇,魏澎.邻联茴香胺-H_2O_2-辣根过氧化物酶伏安酶联免疫分析检测四种重要植物病毒[J].青岛科技大学学报,1998(3). |
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| 作者姓名： | 焦奎 韦璐 张书圣 刘澄凡 张成良 张作芳 刘靖宇 魏澎 |
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| 作者单位： | 青岛化工学院应用化学系，农业部植物检疫实验所，核工业北京地质研究院 |
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| 基金项目： | 国家自然科学基金,山东省自然科学基金 |
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| 摘 要： | 将邻联茴香胺（ＯＤＡ） Ｈ２Ｏ２ 辣根过氧化物酶（ＨＲＰ）伏安酶联免疫分析体系应用于测定南芥菜花叶病毒（ＡｒａｂｉｃＭｏｓａｉｃＶｉｒｕｓ ＡｒＭＶ）、黄瓜花叶病毒（ＣｕｃｕｍｂｅｒＭｏｓａｉｃＶｉｒｕｓ ＣＭＶ）、南方菜豆花叶病毒（ＳｏｕｔｈｅｒｎＢｅａｎＭｏｓａｉｃＶｉｒｕｓ ＳＢＭＶ）和芜菁花叶病毒（ＴｕｒｎｉｐＭｏｓａｉｃＶｉｒｕｓ ＴｕＭＶ）等四种重要植物病毒，其灵敏度高于酶联免疫吸附显色光度法（ＥＬＩＳＡ）。
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| 关 键 词： | 南芥菜花叶病毒 黄瓜花叶病毒 南方菜豆花叶病毒 芜菁花叶病毒 伏安酶联免疫分析 |
Detection of Four Important Plant Viruses with Voltammetric Enzyme Linked Immunoassay Based on ODA H 2O 2 HRP System |
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| Jiao Kui Wei Lu,Zhang Shusheng Liu Chengfan.Detection of Four Important Plant Viruses with Voltammetric Enzyme Linked Immunoassay Based on ODA H 2O 2 HRP System[J].Journal of Qingdao University of Science and Technology:Natutral Science Edition,1998(3). |
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| Authors: | Jiao Kui Wei Lu Zhang Shusheng Liu Chengfan |
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| Abstract: | Arabic mosaic virus(ArMV),cucumber mosaic virus(CMV),southern bean mosaic virus(SBMV)and turnip mosaic virus(TuMV) have been detected by voltammetric enzyme linked immunoassay based on o dianisidine(ODA) H 2O 2 horseradish peroxidase(HRP) system.The highest dilution ratios to detect the leaf saps infected by ARMV,CMV,SBMV and TuMV were :1∶1×10 5,1∶2.5×10 4,1∶2.5×10 6 and 1∶5×10 5,and the detection ranges 1∶10～1∶1×10 5,1∶10～1∶2.5×10 4,1∶5×10 2～1∶2.5×10 6and 1∶2.5×10 3～1∶5×10 5,respectively. The sensitivity of the presented method was higher than that of the enzyme linked immunosorbent assay(ELISA) method. |
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| Keywords: | arabic mosaic virus cucumber mosaic virus southern bean mosaic virus turnip mosaic virus voltammetric enzyme linked immunoassay |
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