| 抗乙硫氨酸突变株产蛋氨酸发酵工艺条件的优化 |
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| 引用本文： | 林小秋,冯琦,张东环,闵伟红.抗乙硫氨酸突变株产蛋氨酸发酵工艺条件的优化[J].食品科学,2009,30(3):243-248. |
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| 作者姓名： | 林小秋 冯琦 张东环 闵伟红 |
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| 作者单位： | 吉林农业大学食品科学与工程学院 |
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| 摘 要： | 本实验研究了抗乙硫氨酸突变株E31 营养条件和培养条件的优化，结果表明：突变株E31 发酵产蛋氨酸营养条件的最佳配方是葡萄糖11%、尿素0.8%、MgSO4·7H2O 0.08%、生物素3μg/100ml，最佳培养条件组合是发酵培养基pH6、发酵温度33℃、接种量8%、发酵时间84h，在此条件下蛋氨酸产量为2.086g/L，比优化前蛋氨酸产量(1.479 g/L)提高了0.607g/L。
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| 关 键 词： | 蛋氨酸 抗乙硫氨酸突变株 发酵 |
| 收稿时间： | 2008-10-29 |
Optimization of Fermentation Conditions for Methionine Production by Ethionine-resistant Mutant |
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| LIN Xiao-qiu,FENG Qi,ZHANG Dong-huan,MIN Wei-hong.Optimization of Fermentation Conditions for Methionine Production by Ethionine-resistant Mutant[J].Food Science,2009,30(3):243-248. |
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| Authors: | LIN Xiao-qiu FENG Qi ZHANG Dong-huan MIN Wei-hong |
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| Affiliation: | (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China) |
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| Abstract: | This study aimed to optimize the conditions of nutrition and cultivation of ethionine-resistant mutant E31. The results showed the best nutrition conditions are 11% glucose, 0.8% urea, 0.08% MgSO4·7H2O, 3 μg /100ml biotin, the best cultivation conditions are pH 6,fermentation temperature 33℃, inoculation size 8% and fermentation time 84 h, After the opimization, the yield of methionine is 2.086 g/L, 0.607g/L higher than that before the optimization. |
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| Keywords: | methionine ethionine resistant mutant fermentation |
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