铕原子标记技术检验实验室移液器容量误差方法

建立铕原子标记技术对实验室移液器容量的比对与校准,能实现对小容量移液器容量准确度与精密度的有效监控。采用时间分辨荧光检测技术,检验移液器容量的误差来源。时间分辨荧光法检测批内重变性误差在0.17%~7.8%之间。铕标记法与蒸馏水称重法对移液器容量测定的比对试验,相对误差-3.9%~0.62%。时间分辨荧光进行移液器容量比对与误差评估,方法灵敏、精确、简便,可适用于临床实验室移液器容量的误差识别及常规比对。

Evaluation of pipettot perforance verified deviation by europium-labeled

To establish a method for laboratoriaI pipettor＇s volume checking and calibration, which effective monitoring the precision and accuracy for the dispeners of the micro pipettor volume. Using timeresolved fiuoroimmunoassay （TR-FIA）, Europium atoms as a labeling material, β -naplatlaoyltrifluoroacetone as intensifier for fluorescence, wavelength between 336 to 337um to explode and between 610 to 613mm to measure, fluorescent intensity as observational mark to verify the source of pipettor＇s volume deviation. In the group of TR-FIA, the inter-assay imprecision were 0.17%-7.8%; The data were steady when measurd at once and after 15min, 30min, 60min. Comparing the results of EuropiumAabeled method with distilled water gravimetric method, which shows that the relative deviationwere -3.9%-0.62%. TR-FIA for pipettor volume comparing and valuation of deviation are sensitive, precise and convenaent. ＇Ibis technique can be used for verified deviation and routine comparing of the pipettor＇s volume in clinical laboratory.