Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment |
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| Authors: | Ana R Rama Francisco Quionero Cristina Mesas Consolacin Melguizo Jose Prados |
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| Affiliation: | 1.Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM), University of Granada, 18016 Granada, Spain; (F.Q.); (C.M.); (C.M.); (J.P.);2.Institute of Biosanitary Research from Granada (ibs. GRANADA), 18012 Granada, Spain;3.Department of Health Sciences, Faculty of Health Sciences, University of Jaen, 23071 Jaen, Spain;4.Department of Anatomy and Embryology, Faculty of Medicine, University of Granada, 18016 Granada, Spain |
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| Abstract: | Lung cancer is the most common cancer in the world and several miRNAs are associated with it. MiRNA sponges are presented as tools to inhibit miRNAs. We designed a system to capture miRNAs based on circular RNAs (circRNA). To demonstrate its usefulness, we chose miR-21, which is upregulated and implicated in lung cancer. We constructed a miR-21 sponge and inserted it into a vector that facilitates circular RNA production (Circ-21) to study its effect on growth, colony formation, and migration in lung cancer cell lines and multicellular tumor spheroids (MTS). Circ-21 induced a significant and time-dependent decrease in the growth of A549 and LL2 cells, but not in L132 cells. Furthermore, A549 and LL2 cells transfected with Circ-21 showed a lower number of colonies and migration than L132. Similar findings were seen in A549 and LL2 Circ-21 MTS, which showed a significant decrease in volume growth, but not in L132 Circ-21 MTS. Based on this, the miR-21 circular sponge may suppress the processes of tumorigenesis and progression. Therefore, our system based on circular sponges seems to be effective, as a tool for the capture of other miRNAs. |
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| Keywords: | miRNA circular sponges miRNA-21 lung cancer |
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