Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I |
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| Authors: | Helen Bergquist Raviteja Inturi Rula Zain Tanel Punga |
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| Affiliation: | 1.Department of Medical Biochemistry and Microbiology, Uppsala University, BMC Box 582, Husargatan 3, SE-751 23 Uppsala, Sweden; (H.B.); (R.I.);2.Department of Laboratory Medicine, Translational Research Center Karolinska (TRACK), Karolinska Institutet, Huddinge, SE-141 86 Stockholm, Sweden;3.Centre for Rare Diseases, Karolinska University Hospital, SE-171 76 Stockholm, Sweden |
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| Abstract: | RNA molecules can adopt specific RNA triplex structures to execute critical biological functions. Human adenoviruses (HAdVs) are abundant pathogens encoding the essential, noncoding virus-associated RNA I (VA RNAI). Here, we employ a triplex-specific probing assay, based on the intercalating and cleaving agent benzoquinoquinoxaline 1, 10-phenanthroline (BQQ–OP), to unravel a potential RNA triplex formation in VA RNAI. The BQQ–OP cleavage of the pathogenic HAdV type 4 (HAdV-4) VA RNAI indicates that a potential triplex is formed involving the highly conserved stem 4 of the central domain and side stem 7. Further, the integrity of the HAdV-4 VA RNAI side stem 7 contributes to a potential triplex formation in vitro and virus growth in vivo. Collectively, we propose that the HAdV-4 VA RNAI can potentially form a biologically relevant triplex structure. |
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| Keywords: | adenovirus, VA RNAI, triplex RNA, BQQ– OP |
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