| 检测赭曲霉毒素A(OTA)的酶联免疫吸附法(ELISA)体系的建立 |
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| 引用本文： | 熊勇华,陈雪岚,许杨. 检测赭曲霉毒素A(OTA)的酶联免疫吸附法(ELISA)体系的建立[J]. 食品科学, 2006, 27(5): 30-35 |
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| 作者姓名： | 熊勇华 陈雪岚 许杨 |
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| 作者单位： | 南昌大学食品科学教育部重点实验室； 江西师范大学生命科学学院； |
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| 基金项目： | 江西省科技厅农业重点项目 |
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| 摘 要： | 本文分别以自制抗-OTA兔血清抗体(IgG)及鸡卵黄抗体(IgY)就影响检测OTA的ELISA(酶联免疫吸附法)体系因素进行了探讨,建立了检测OTA的ELISA方法。鸡卵黄抗体的最佳ELISA操作参数为:包被抗原浓度为7.5μg/ml,封阻剂浓度为2.5%,抗体稀释倍数为1000,酶标二抗稀释倍数为14000;兔血清抗体的最佳ELISA操作参数为:包被抗原浓度为2.5μg/ml,封阻剂浓度为2.5%,抗体稀释倍数为8000,酶标二抗稀释倍数为10000。IgG和IgY的检测灵敏度分别为10ng/ml和1ng/ml。
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| 关 键 词： | 赭曲霉素A 酶联免疫吸附法 |
| 文章编号： | 1002-6630（2006）05-0030-06 |
| 收稿时间： | 2005-02-28 |
| 修稿时间： | 2005-02-28 |
Development of ELISA System to Determine Ochratoxin A(OTA) |
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| XIONG Yong-Hua,Chen Xue-Lan,XU Yang. Development of ELISA System to Determine Ochratoxin A(OTA)[J]. Food Science, 2006, 27(5): 30-35 |
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| Authors: | XIONG Yong-Hua Chen Xue-Lan XU Yang |
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| Affiliation: | 1.Key Laboratory of Food Science, Ministry of Education, Nanchang University, Nanchang 330047, China; 2.School of Bioscience, Jiangxi Normal University, Nanchang 330046, China |
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| Abstract: | In the paper, the methods of the indirect competitive ELISA for determining OTA were developed by using self- prepared rabbit serum antibody (IgG) and immunoglublin yolk (IgY). The optimum ELISA parameters with IgY were that the concentration of antigen and blocking agent was 7.5μg/ml, 2.5% and the dilution multiples of IgY and anti-chicken rabbit antibody-HRP were 1000 and 14000, respectively. The optimum parameters with rabbit IgG were that the concentration of antigen and blockaded agent was 2.5μg/ml, 2.5% and the dilution multiples of rabbit IgG and anti-rabbit goat antibody-HRP were 8000 and 10000, respectively. The detection limits of the ELISA with IgY and rabbit IgG were 10ng/ml and 1ng/ml, respectively. |
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| Keywords: | Ochratoxin A(OTA) ELISA |
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