蛋白酶产生菌的筛选及发酵条件的优化 |
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引用本文: | 陈显玲. 蛋白酶产生菌的筛选及发酵条件的优化[J]. 广州化工, 2014, 0(7): 67-70 |
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作者姓名: | 陈显玲 |
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作者单位: | 燕京啤酒(玉林)有限公司,广西玉林537000 |
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摘 要: | 筛选了植物蛋白加工用蛋白酶产生菌,为酶的分子改造和植物蛋白加工提供材料基础。通过不同菌株筛选,获得了一株蛋白酶的高产菌株枯草芽孢杆菌X12。经过单因子实验,确立了产酶的最适培养基配方:蔗糖7.5 g/L,黄豆粉6.0 g/L,MnSO40.04 g/L,初始pH=7.0,装液量75 mL/250 mL。结论:按照所优化的产酶条件于37℃,180 r/min,经60 h培养,发酵液最终酶活达1 868.4 u/mL。
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关 键 词: | 蛋白酶 筛选 枯草芽孢杆菌 优化 |
Optimization of Screening of Protease Producing Strain and the Fermentation Condition |
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Affiliation: | CHEN Xian - ling (Yan Jing Beer (Yulin) Co., Ltd., Guangxi Yulin 537000, China) |
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Abstract: | To obtain high and process to produce poly- peptide. well defined By repeated s protease -producing strains, these strains can be used in plant protein iflings of bcterial strains, a productive protease bacterial strain Bacillus subtilis X12 was found. Through single - factor test, the best formula for the substratum obtained was sucrose 7.5 g/L, soybean meal 6. 0 g/L, MnSO4 0.04 g/L, pH 7.0, fluid volume 75 mL and 250 mL. Under the conditions of temperature 37 ℃, 180 r/min and 60 h, the enzymatic activity of the final fermentation liquid reached 1868.4 u/mL. |
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Keywords: | protease screening Bacillus subtilis optimization |
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