| 65株冷冻肠衣分离细菌的鉴定 |
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| 引用本文： | 仇保丰,宋鸿雁,蔡宝亮,邵义祥,顾炳泉,刘文斌,高逢结,李建. 65株冷冻肠衣分离细菌的鉴定[J]. 中国食品卫生杂志, 2013, 25(5): 458-461 |
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| 作者姓名： | 仇保丰 宋鸿雁 蔡宝亮 邵义祥 顾炳泉 刘文斌 高逢结 李建 |
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| 作者单位： | 南通出入境检验检疫局,江苏 南通 226004;南通大学实验动物中心,江苏 南通 226001;江苏出入境检验检疫局,江苏 南京 210001;南通大学实验动物中心,江苏 南通 226001;南通出入境检验检疫局,江苏 南通 226004;南通出入境检验检疫局,江苏 南通 226004;南通出入境检验检疫局,江苏 南通 226004;南通出入境检验检疫局,江苏 南通 226004 |
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| 基金项目： | 南通市科技项目(BK2012091)；江苏检验检疫局科研项目(2010KJ35) |
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| 摘 要： | 调查冷冻肠衣携带细菌的情况。方法用16S rDNA PCR序列扩增和分析技术对2010—2012年从南通地区肠衣生产、加工企业的冷冻肠衣中分离的65株细菌进行鉴定。结果 这些细菌包含了来自13个属的20种细菌,其中奇异变形杆菌(Proteus mirabils)、海氏肠球菌(Enterococcus hirae)和粪肠球菌(Enterococcus faecalis)是比较明显的优势种群。另外,许多细菌在一定条件下可引起人和动物发生疾病。结论 冷冻肠衣可以携带多种细菌,应该进一步加强监测和研究。
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| 关 键 词： | 冷冻肠衣 16S rDNA 食源性致病菌 |
| 收稿时间： | 2013-06-18 |
Identification of 65strains of bacteria isolated from frozen intestines |
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| QIU Bao-feng,SONG Hong-yan,CAI Bao-liang,SHAO Yi-xiang,GU Bing-quan,LIU Wen-bin,GAO Feng-jie and LI Jian. Identification of 65strains of bacteria isolated from frozen intestines[J]. Chinese Journal of Food Hygiene, 2013, 25(5): 458-461 |
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| Authors: | QIU Bao-feng SONG Hong-yan CAI Bao-liang SHAO Yi-xiang GU Bing-quan LIU Wen-bin GAO Feng-jie LI Jian |
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| Affiliation: | (Nantong Entry-Exit Inspection and Quarantine Bureau,Jiangsu Nantong 226004,China) |
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| Abstract: | To study the bacteria contamination in frozen intestines.Methods Sixty-five strains of bacteria isolated from frozen intestines, which collected in Nantong casings manufacturing and processing enterprises between 2010and 2012, were identified by 16S rDNA PCR amplification and sequence analysis.Results It showed that 20specices of bacteria blonging to 13genuses were identified,and the main bacterial species were Proteus mirabils, Enterococcus hirae and Enterococcus faecalis.In addition, some of them may cause disease in humans and animals under certain conditions.Conclusion A variety of bacteria may be carried by frozen intestines, the relevant supervision and study should be further strengthened. |
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