| Fe 3O 4-COOH纳米材料对大肠杆菌菌株水平的指纹峰确定 |
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| 引用本文： | 黄艳玲,程杨,李春媛.Fe 3O 4-COOH纳米材料对大肠杆菌菌株水平的指纹峰确定[J].食品与生物技术学报,2020,39(12):80-84. |
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| 作者姓名： | 黄艳玲 程杨 李春媛 |
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| 作者单位： | 天津现代职业技术学院 生物工程学院，天津300350;天津市食品研究所有限公司，天津301609;国家农产品保鲜工程技术研究中心(天津) 农业部农产品贮藏保鲜重点实验室，天津 300384 |
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| 摘 要： | 通过合成Fe 3O 4COOH纳米颗粒提高MALDI-TOF MS对大肠杆菌菌株水平的鉴别，并利用Fe 3O 4-COOH纳米对大肠杆菌K-12和大肠杆菌DSM 30083T裂解液中的菌体蛋白进行富集，不仅增加MALDI-TOF MS检测的分辨率和灵敏性，而且可以大大增加大肠杆菌菌株水平的指纹峰，根据实验结果，大肠杆菌K-12有4个匹配的指纹峰，而大肠杆菌DSM 30083T获得8个指纹峰，从而提高MALDI-TOF MS对大肠杆菌菌株水平的鉴别。
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| 关 键 词： | 富集 细菌 MALDI-TOF MS 鉴别 |
Identification of Fingerprint Peaks at the Level of E. coli Strains via Fe3O4-COOH Nanomaterials |
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| HUANG Yanling,CHENG Yang,LI Chunyuan.Identification of Fingerprint Peaks at the Level of E. coli Strains via Fe3O4-COOH Nanomaterials[J].Journal of Food Science and Biotechnology,2020,39(12):80-84. |
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| Authors: | HUANG Yanling CHENG Yang LI Chunyuan |
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| Affiliation: | School of Biotechnology, Tianjin Modern Vocational Technology College, Tianjin 300350,China;Tianjin Food Research Institute Co., Ltd., Tianjin 301609, China; National Engineering Technology Research Center for Preservation of Agricultural Products(Tianjin), Key Laboratory of Storage of Agricultural Products, Ministry of Agriculture and Rural Affairs, Tianjin 300384, China |
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| Abstract: | Identification of E. coli strain by MALDI-TOF MS was improved by synthesis of Fe 3O 4-COOH nanomaterials. The fingerprint peaks of E. coli strain, as well as the resolution and sensitivity of MALDI-TOF MS detection were increased by the enrichment of bacterial proteins in E. coli K-12 and DSM 30083T lysate. Results showed that E. coli K-12 had four matching peaks, while E. coli DSM 30083T had eight peaks, which thus improved the identification of E. coli strains by MALDI-TOF MS. |
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| Keywords: | enrichment bacterium MALDI-TOF MS identification |
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