超高效液相色谱法同时测定保健品中4种大豆异黄酮

目的：建立超高效液相色谱法同时测定保健品中大豆苷(Daidzin)、染料木苷(Genistin)、染料木素(Daidzein)和大豆素(Genistein)4种大豆异黄酮。方法：样品用80%甲醇超声提取后,以甲醇-0.1%磷酸水(V/V)作为流动相梯度洗脱分离,使用二极管阵列检测器(Photo-diode Array, PDA)于260 nm波长下测定。结果：4种大豆异黄酮在1.0~50 mg/L范围内,标准曲线线性良好,相关系数R²均大于0.999。平均回收率为86.7%~106%,相对标准偏差RSDs为1.31%~6.32%。方法检出限为0.5~1.0 mg/kg,方法定量限为1.5~3.0 mg/kg。结论：方法学实验结果表明,该方法回收率高,灵敏度高,且操作简单,快速高效,可应用于实际样品测定。

Simultaneous determination of 4 kinds of soybean isoflavones in health products by ultra performance liquid chromatography

Objective : An ultra performance liquid chromatography method was established for determination of four soybean isoflavones in health products included daidzin, genistin, daidzein and genistein. Method: Samples were ultrasonic extracted by 80% methanol-water, then separated by methanol-0.1% phosphoric acid-water (V/V) as mobile phase with gradient elution, finally detected by photo-diode array detector under the wavelength of 260 nm. Results : The standard curve of four soybean isoflavones exhibited a good linearity in the concentration range of 1.0-50 mg/L, while the correlation coefficient (R²) were both greater than 0.999. Moreover, the average recoveries were ranged from 86.7% to 106% with the relative standard deviations were ranged from 1.31% to 6.32%. The limits of detection and the limits of quantitation were 0.5-1.0 mg/kg and 1.5-3.0 mg/kg respectively. Conclusion : Methodological experiment results indicated that this method had high recovery rate and high sensitivity . In addition, it was simple operation, fast and efficient that could be applied to actual determination.