水酶法提取山苍子核仁油工艺的研究

山苍子是我国特有的木本油料资源,目前主要利用成分为果实精油.为了充分开发山苍子核仁资源,用水酶法提取山苍子核仁油,以山苍子核仁为原料,考察酶的种类及复配、酶用量、酶解时间等对核仁油提取率的影响;在单因素实验的基础上,利用响应面中心组合(Box-Behnken)实验设计对酶解反应的温度、液料比、pH进行工艺参数优化,并对...

Study on Extraction Technology of Litsea Cubeba Kernel Oil by Aqueous Enzymatic Method

Litsea cubeba is a unique woody oil resource in China, and the main ingredient is fruit essential oil. In order to make full use of the resources of Litsea Cubeba kernel and optimize the process for extracting the Litsea Cubeba kernel oil by aqueous enzymatic method. Using the kernel of Litsea Cubeba as raw material, the effects of the types and compounding of enzymes, the amount of enzymes and the reaction time on the extraction rate of kernel oil were studied. Based on the single factor experiment, the Box-Behnken experimental design was used to optimize the process parameters of the temperature, liquid-material ratio, and pH of the enzymatic hydrolysis reaction, and the Litsea Cubeba kernel oil extracted under the best process conditions was analyzed by FT-IR and GC-MS. The results showed that the best composite enzyme preparation of Litsea Cubeba kernel oil by aqueous enzymatic method was the mass ratio of neutral protease to cellulase 1:1, the amount of complex enzyme was 3%, the enzymolysis time was 7h, Liquid-solid ratio 3 mL/g, pH 6.40, and temperature 46°C. Under these conditions, the extraction rate of Litsea cubeba kernel oil reached (86.10±0.68) %. FT-IR detected OH, C=O, CH3, CH2, C=O, CO functional groups, fatty acids containing these functional groups were found in GC-MS analysis. GC-MS identified 5 kinds of saturated fatty acids and 6 kinds Unsaturated acid fatty acid. Different oil extraction processes had no significant effect on fatty acids.