| HPLC-RI测试果葡糖浆中的果糖和葡萄糖 |
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| 引用本文： | 杨晓燕,刘玉莲,张伟,李小侠. HPLC-RI测试果葡糖浆中的果糖和葡萄糖[J]. 山东化工, 2011, 40(1): 46-48 |
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| 作者姓名： | 杨晓燕 刘玉莲 张伟 李小侠 |
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| 作者单位： | 江苏天瑞仪器股份有限公司,江苏,苏州,215300 |
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| 摘 要： | 建立并验证了用高效液相色谱-示差折光检测器测定果葡糖浆中果糖和葡萄糖含量的检测方法。以水为溶剂,Ca型阳离子交换柱进行分离;以相对保留时间定性,色谱峰面积定量。方法平均回收率为98.33%~102.69%,RSD为0.865%~1.253%,检测限(S/N=3)分别为葡萄糖1.94μg/mL;果糖2.49μg/mL。实验表明该方法对果葡糖浆中的葡萄糖和果糖含量的测试简单、可靠。
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| 关 键 词： | 示差折光检测器 果葡糖浆 Ca型阳离子交换柱 |
Determination of Glucose and Fructose in High Fructose Corn Syrup by HPLC-RI |
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| YANG Xiao-Yan,LIU Yu-Lian,ZHANG Wei,LI Xiao-Xia. Determination of Glucose and Fructose in High Fructose Corn Syrup by HPLC-RI[J]. Shandong Chemical Industry, 2011, 40(1): 46-48 |
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| Authors: | YANG Xiao-Yan LIU Yu-Lian ZHANG Wei LI Xiao-Xia |
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| Affiliation: | YANG Xiao -Yan, LIU Yu - Lian, ZHANG Wei, LI Xiao - Xia (Jiangsu Skyray Instrument Co., Ltd. , Suzhou 215300, China) |
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| Abstract: | Established and tested the glucose and fructose in fructose corn syrup using HPLC - RI. Using water as solvent, cation exchange column with Ca type for separate;qualitative with relatively retention time and quantitative with peak area. The average recovery rate was 98.33% - 102.69%, RSD was 0.994% - 1. 377%, and the limit of detection（S/N =3） respectively as glucose 1. 94μg/mL;fructose 2. 49μg/mL. The experiment showed that this method was simple and reliable for the control of glucose and fructose which in high fructose corn syrup. |
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| Keywords: | RI detector high fructose corn syrup cation exchange column with Ca type |
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