纳他霉素的大孔树脂原位吸附动力学研究

考察9种大孔树脂对纳他霉素的静态吸附行为和解析效果,探讨吸附过程中树脂静态吸附动力学和等温吸附过程,并研究大孔树脂在Streptomyces natalensis HW-2发酵液中对纳他霉素的原位吸附效果。结果表明,HPD300和HPD450树脂对纳他霉素的吸附与解吸效果较好,吸附率分别为83.47%,96.14%,解吸率分别为95.22%,85.42%,吸附量分别达到0.63,0.72mg/g干树脂,HPD450适合对纳他霉素的吸附和解析。根据HPD450树脂的等温吸附动力学,HPD450树脂对纳他霉素的吸附符合Langmuir方程,主要为单分子层吸附。在发酵48h时添加4g/30mL的HPD450树脂进行原位吸附,使发酵过程中纳他霉素总产量提高了66.27%。

In-situ adsorption kinetics of macroporous resin on natamycin

In this study, the static adsorption behavior and desorption effects of 9 kinds of macroporous resins on natamycin were investigated. The static adsorption kinetics and isothermal adsorption process of the resins were discussed. The in-situ adsorption effects of the macroporous resins on natamycin were also studied in Streptomyces natalensis HW-2 fermentation broth. The results showed that the adsorption and desorption effects of HPD300 and HPD450 resins on natamycin were better, the adsorption rates were 83.47% and 96.14%, respectively. The desorption rates were 95.22% and 85.42%, respectively, and the adsorption capacity was 0.63 and 0.72 mg/g dry resins, respectively. HPD450 was more suitable for the adsorption and analysis of natamycin. According to the Langmuir and Freundlich parameters of isothermal adsorption of HPD450 resin, the adsorption of HPD450 resin conformed to Langmuir equation and was mainly monolayer adsorption. With the Addition of 4 g/30 mL HPD450 resin in 48 hours of fermentation to the fermentation broth for in-situ adsorption, the total yield of natamycin was increased by 66.27%.