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海藻糖合酶产生菌基因组DNA的提取方法
引用本文:刘巧红,马莺. 海藻糖合酶产生菌基因组DNA的提取方法[J]. 中国甜菜糖业, 2007, 0(2): 8-9
作者姓名:刘巧红  马莺
作者单位:哈尔滨工业大学食品科学与工程学院,黑龙江,哈尔滨,150090
摘    要:以海藻糖合酶产生菌——恶臭假单胞菌为目标菌,建立了一种快速、简便提取假单胞菌染色体DNA的方法,利用本方法提取的DNA结构完整、无降解、纯度较高,无需纯化,可直接作为PCR方法扩增目的基因的模板。

关 键 词:恶臭假单胞菌  基因组DNA  DNA提取
文章编号:1002-0651(2007)02-0008-02
修稿时间:2006-09-21

Rapid extracting approach of the genomio DNA from producing trehalose synhase strain
Liu Qiao-hong,Ma Ying. Rapid extracting approach of the genomio DNA from producing trehalose synhase strain[J]. China Beet & Sugar, 2007, 0(2): 8-9
Authors:Liu Qiao-hong  Ma Ying
Affiliation:Institute of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China
Abstract:A rapid and simple approach in the genomic DNA extracting from the pseudomonas putida 1. 1819 which can produce trehalose synthase was promoted in this paper. This method was both easier and economical. Results showed that DNA isolated with this method was intergrated and purely. The purity of the obtained DNA was good enough for the PCR analysis experiment.
Keywords:pseudomonas putida   genome   DNA extraction
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