利用表面等离子体共振技术检测猪尿中盐酸克伦特罗

利用表面等离子体共振技术检测猪尿液中的盐酸克伦特罗。将盐酸克伦特罗-牛血清白蛋白偶联物作为传感芯片特异识别探针结合到生物芯片表面,通过溶液竞争法结合表面等离子体共振技术检测盐酸克伦特罗。标准曲线被建立并且检测盐酸克伦特罗的检测限能够达到1.03 ng/m L,与莱克多巴胺、沙丁胺醇无交叉反应,具有良好的特异性,猪尿的回收率为98.2%~107.0%,变异系数在可接受范围内。所采用的表面等离子体共振方法对于检测猪尿中的盐酸克伦特罗具有灵敏性,准确性,精密性。

Detection of Clenbuterol Hydrochlorid in Swine Urine Using Surface Plasmon Resonance

Surface plasmon resonance technique was used to detect clenbuterol hydrochlorid （CLB） in swine urine. clenbuterol hydrochlorid-bovine serum albumin conjugate （CLB-BSA） as a specific recognition probe was bound to the surface of the biological chip, and the solution competition method combined with surface Plasmon resonance technique was used to detect CLB .The calibration curve was established and the limit of detection was 1.03 ng/mL. No cross reaction with ractopamine or salbutamol was found as well as good specificity. The recoveries of CLB from spiked samples were in the range of 98.2%-107.0%, with acceptable coefficients of variation. The proposed SPR method proved to be sensitive, accurate and precise.