| 聚乙二醇/硫酸铵双水相体系萃取葡萄糖氧化酶的研究 |
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| 引用本文： | 陈利梅,李德茂,孙秀平.聚乙二醇/硫酸铵双水相体系萃取葡萄糖氧化酶的研究[J].食品工业科技,2010(5). |
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| 作者姓名： | 陈利梅 李德茂 孙秀平 |
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| 作者单位： | 聊城大学农学院食品系,山东聊城,252059 |
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| 摘 要： | 采用聚乙二醇/硫酸铵PEG/(NH4)2SO4]双水相体系对葡萄糖氧化酶的萃取进行了研究。以双水相系统的上、下相的酶活、比酶活、相比R、分配系数K和萃取率Y等为参数,探讨了PEG质量分数、不同PEG浓度和不同硫酸铵浓度对葡萄糖氧化酶在PEG/(NH4)2SO4双水相系统中分配行为的影响。实验结果表明:葡萄糖氧化酶在25%的PEG4000和10%硫酸铵的双水相体系中可获得较高的萃取率达81.11%,分配系数为0.134。
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| 关 键 词： | 葡萄糖氧化酶 双水相 聚乙二醇 硫酸铵 |
Extraction of glucose oxidase by PEG/(NH4)2SO4 aqueous two-phase systems |
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| CHEN Li-mei,LI De-mao,SUN Xiu-ping.Extraction of glucose oxidase by PEG/(NH4)2SO4 aqueous two-phase systems[J].Science and Technology of Food Industry,2010(5). |
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| Authors: | CHEN Li-mei LI De-mao SUN Xiu-ping |
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| Affiliation: | CHEN Li-mei,LI De-mao,SUN Xiu-ping(Food Science Department,Liaocheng University,Liaocheng 252059,China) |
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| Abstract: | Effects of the PEG molecular weight,PEG concentration and ammonium sulfate concentration on the glucose oxidase enzyme activity in the PEG/(NH4)2SO4 aqueous two-phase system were studied according to parameters of enzyme activity,R,partition coefficient K and the extraction rate Y. The results indicated that the best PEG molecular weight was 4000,the best PEG 4000 concentration was 25%,and the best ammonium sulfate concentration was 10%. Under above conditions,the partition coefficient K was 0.134 and the e... |
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| Keywords: | glucose oxidase aqueous two-phase systems PEG (NH4)2SO4 |
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