| 豆壳过氧化物酶的分离纯化新工艺研究 |
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| 引用本文： | 刘均洪,邱龙辉,李俊峰,刘全兰,王繁业,吴汝林. 豆壳过氧化物酶的分离纯化新工艺研究[J]. 化学工程, 2005, 33(6): 31-34 |
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| 作者姓名： | 刘均洪 邱龙辉 李俊峰 刘全兰 王繁业 吴汝林 |
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| 作者单位： | 青岛科技大学,生物与制药工程系,山东,青岛,266042;青岛科技大学,生物与制药工程系,山东,青岛,266042;青岛科技大学,生物与制药工程系,山东,青岛,266042;青岛科技大学,生物与制药工程系,山东,青岛,266042;青岛科技大学,生物与制药工程系,山东,青岛,266042;青岛科技大学,生物与制药工程系,山东,青岛,266042 |
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| 基金项目： | 国家自然科学基金资助项目（20176019） |
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| 摘 要： | 采用一种新的简单纯化工艺进行了过氧化物酶分离纯化实验研究。该工艺为酶液经硫酸铵-丙酮协同沉淀,丙酮分级沉淀,最后用硫酸锌除杂。使豆壳过氧化物酶的Rz达到1.32,总酶活收率为65%。使用了丙酮-硫酸铵协同沉淀法,即有机溶剂-无机盐二相系统,是一种简便有效的分离纯化生物大分子的新方法。
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| 关 键 词： | 大豆过氧化物酶 纯化 协同沉淀 丙酮分级沉淀 |
| 文章编号： | 1005-9954（2005）06-0031-04 |
A novel process of purifying soybean hull peroxidase |
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| LIU Jun-hong,QIU Long-hui,LI Jun-feng,LIU Quan-lan,WANG Fan-ye,WU Ru-Lin. A novel process of purifying soybean hull peroxidase[J]. Chemical Engineering, 2005, 33(6): 31-34 |
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| Authors: | LIU Jun-hong QIU Long-hui LI Jun-feng LIU Quan-lan WANG Fan-ye WU Ru-Lin |
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| Abstract: | The purification method of peroxidase was studied. A novel and simple purification process was used. R2 value of soybean peroxidase reached 1.32 and the recovery of enzyme activity was 65% after purification. In this new process, the enzyme solution was purified by the ammonium sulphate-acetone cooperation precipitation, the acetone fractional precipitation and zinc sulfate precipitation. The ammonium sulphate-acetone cooperation precipitation, namely, the system of organic solvent-inorganic salt two phases, was adopted in the process. This is a simple and effective purification method of biomacromolecule. |
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| Keywords: | soybean hull peroxidase(SHP) purification cooperation precipitation acetone fractional precipitation |
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