微胶囊化儿茶素对阿霉素肾病大鼠总抗氧化能力的影响

目的:探讨微胶囊化儿茶素对阿霉素肾病大鼠总抗氧化能力的影响。方法:将60只雌性SD大鼠随机分为对照组、肾病组、激素组、VE组、儿茶素组和微胶囊组共六组,尾静脉一次性注射阿霉素(5mg/kg)制备肾病模型;实验第六周末杀鼠取尿、血、肾及肝脏,利用生化法测定血、肾及肝脏总抗氧化能力和·OH,利用考马斯亮蓝法测定24h尿蛋白的排泄量。结果:微胶囊组大鼠血、肾及肝脏中总抗氧化能力显著高于儿茶素组(p<0.01);微胶囊组大鼠·OH的浓度在肾与肝脏中显著低于儿茶素组(p分别<0.05,0.01),在血清中无显著性差异;微胶囊化儿茶素治疗组24h尿蛋白排泄量显著低于儿茶素治疗组(p>0.05)。结论:微胶囊化儿茶素可能是通过提高肾病大鼠总抗氧化能力,有效清除·OH,达到降低肾病大鼠尿蛋白排泄的目的。

Effect of Catechin Microcapsule on Total Antioxidation Competence in Rats with Nephritic Adriblastine Induced Syndrome

Objective: To study the effect of catechin microcapsule on total antioxidation competence in rats with adriblastine induced nephritie syndrome. Methods: 60 female SD rats are random distributed as control group, nephritic syndrome group, glucocorticoid group, vitamin E group, catechin group and microcapsule group. Catechin microcapsule is made by the way of mist spray dehydration. Nephritic syndrome rats are caused by injecting adriblastin before rats are killed. Blood, urine, liver tissue and kidney tissue are collected.· OH concentration and total antioxidation competence are detected by biochemistry assay. 24h urinary protein excretions are detected by Comb’s bright blueness assay. Results: Total antioxidation competence of microcapsule group in serum, liver tissue and kidney tissue is higher than that of in catechin group(p< 0.01). ·OH concentrations of microcapsule group in liver tissue and kidney tissue respectively are lower than those of catechin group(p<0.05, 0.01 respectively). There is no marked difference in serum. 24h urinary protein excretion in microcapsule group is lower than that of in catechin group(p<0.05). Conclusion: Catechin microcapsule can successfully reduce 24h urinary protein excretion in rats of adriblastine induced nephrotic syndrome and may have achieved through enhancing total antioxidation competence and depressed ·OH concentration.