Selective retrieval of microparticles in microchambers using electrolytically generated bubbles for cell array applications |
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| Authors: | Tomoaki KurakazuAuthor VitaeKaori Kuribayashi-ShigetomiAuthor Vitae Yukiko T MatsunagaAuthor VitaeHiroshi KimuraAuthor Vitae Teruo FujiiAuthor VitaeYasuyuki SakaiAuthor Vitae Shoji TakeuchiAuthor Vitae |
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| Affiliation: | Center for International Research on Micronano Mechatronics, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan |
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| Abstract: | This paper describes a method for the selective retrieval of microparticles using bubbles generated by electrolysis. Microparticles (polystyrene beads, mouse embryos, and cell spheroids) were arrayed in microchambers fabricated in SU-8 on the surface of an electrode consisting of indium tin oxide (ITO) patterned on glass. Bubbles were selectively generated in a target microchamber by applying a voltage to electrodes positioned in the microchamber. As a result, we successfully retrieved microparticles (100 μm in diameter) positioned in the microchambers. This method is gentle enough to maintain cellular viability, and therefore, it will be a powerful tool for the quantitative analysis of cells in an arrayed system. |
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| Keywords: | Selective retrieval Bubble Electrolysis Microfluidic device Cell manipulation |
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