壳聚糖复合不对称膜的制备及生物组织相容性研究

用分步流延干燥法制备了一种由壳聚糖（CS）、聚乙烯醇（PVA）、羧甲基壳聚糖（CM-CS）构成的新型人工皮肤医用材料——壳聚糖复合不对称膜（C-P-C膜），其上层为CS层，中间为PVA层，下层为CM-CS层。研究结果表明，可通过调节CS、PVA、CM-CS的加入量来控制复合不对称膜各层的厚度和膜的水蒸气通透性，C-P-C膜具有良好的透光性和吸水率。生物相容性实验发现，C-P-C膜是无毒材料，不会引起创伤感染，随着植入C-P-C膜时间的延长，组织炎症反应明显减轻，6周时，炎症浸润程度低于手术缝合线。C-P-C膜能够明显地促进创面愈合，能有效地密封出血创面且具有显著的止血作用。

Preparation and histocompatibility analysis of composite asymmetric chitosan membrane modified by blending with carboxymethyl-chitosan

A novel composite asymmetric chitosan membrane(C-P-C) was prepared with casting method and evaluated as an artificial skin iatric-material. In this membrane, chitosan (CS) was the top-layer and carboxymethyl-chitosan (CM-CS) was the sub-layer separated by polyvinyl alcohol (PVA) layer. C-P-C membrane can control water vapor evaporation, and has excellent light transmittance and fluid drainage ability. The tissue-compatible experiment indicated that C-P-C membrane does not possess antigenicity and toxicity, and does not infect a wound with disease germs. The C-P-C membrane is hemostatic and the wound covered with it can be healed quickly. The results show that C-P-C membrane has excellent histocompatibility.