| 四川烟草主栽区根际促生菌筛选及促生菌系构建 |
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| 引用本文： | 吴翔,甘炳成,唐亚,谭昊,黄忠乾,谢丽源,彭卫红,唐杰,周洁,陈影. 四川烟草主栽区根际促生菌筛选及促生菌系构建[J]. 烟草科技, 2019, 0(3): 1-9 |
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| 作者姓名： | 吴翔 甘炳成 唐亚 谭昊 黄忠乾 谢丽源 彭卫红 唐杰 周洁 陈影 |
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| 作者单位： | 四川省农业科学院土壤肥料研究所农业部西南区域农业微生物资源利用科学观测实验站/农业部西南山地农业环境重点实验室;四川大学建筑与环境学院 |
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| 基金项目： | 四川省战略性新兴产业关键核心技术研发项目"秸秆快速腐解技术及复合微生物菌剂研发"(SC2013510104007);四川省财政创新能力提升工程项目"烟草促生与生防复合菌系的构建及应用研究"(2013JCYJ-007) |
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| 摘 要： | 为挖掘四川烟草主栽区根际促生菌资源,采集5个烟草主栽区根际土壤样品,采用稀释平板法分离微生物。通过检测菌株产IAA、铁载体和HCN的能力,从分离的微生物中筛选促生菌并构建促生菌系,检测促生菌系对烟草主栽品种种子发芽率的影响。结果表明,分离的923株微生物中,有5株细菌(YBT-003-B-5、YB-001-B-8、FY-001-B-9、MT-002-B-12、L2-001-B-16)具有较好的促生能力。促生菌系1345(YBT-003-B-5、FY-001-B-9、MT-002-B-12、L2-001-B-16)能显著提高烟草种子的发芽率。16S rDNA序列分析结果表明,菌株YBT-003-B-5、FY-001-B-9、MT-002-B-12分别属于Bacillus tequilensis、Enterobacter xiangfangensi、Klebsiella variicola,菌株L2-001-B-16可能属于肠杆菌科(Enterobacteriaceae)的新类群。
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| 关 键 词： | 四川省 烟草主栽区 促生菌 筛选 发芽率 |
Screening growth-promoting rhizobacteria from main tobacco-growing areas in Sichuan and construction of growth-promoting rhizobacteria groups |
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| WU Xiang,GAN Bingcheng,TANG Ya,TAN Hao,HUANG Zhongqian,XIE Liyuan,PENG Weihong,TANG Jie,ZHOU Jie,CHEN Ying. Screening growth-promoting rhizobacteria from main tobacco-growing areas in Sichuan and construction of growth-promoting rhizobacteria groups[J]. Tobacco Science & Technology, 2019, 0(3): 1-9 |
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| Authors: | WU Xiang GAN Bingcheng TANG Ya TAN Hao HUANG Zhongqian XIE Liyuan PENG Weihong TANG Jie ZHOU Jie CHEN Ying |
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| Affiliation: | (Scientific Observing and Experimental Station of Southwestern Region Agromicrobiological Resource Utilization of Ministry of Agriculture/Key Laboratory of Agricultural Environment in Southwest Mountain Areas of Ministry of Agriculture, Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;College of Architecture and Environment, Sichuan University, Chengdu 610065, China) |
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| Abstract: | WU Xiang;GAN Bingcheng;TANG Ya;TAN Hao;HUANG Zhongqian;XIE Liyuan;PENG Weihong;TANG Jie;ZHOU Jie;CHEN Ying(Scientific Observing and Experimental Station of Southwestern Region Agromicrobiological Resource Utilization of Ministry of Agriculture/Key Laboratory of Agricultural Environment in Southwest Mountain Areas of Ministry of Agriculture, Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;College of Architecture and Environment, Sichuan University, Chengdu 610065, China) |
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| Keywords: | Sichuan Province Main tobacco-growing area Growth-promoting rhizobacterium Screening Germination rate |
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